福建武夷山地区土壤可培养放线菌多样性分析  

Diversity of Culturable Actinomycetes in Soils of Wuyishan Area in Fujian Province

作　　者：吴宝川 陈小尘 黄兆斌 周艳 黄卫红 郑晓婷 薛喜枚 张秋芳 WU Baochuan;CHEN Xiaochen;HUANG Zhaobin;ZHOU Yan;HUANG Weihong;ZHENG Xiaoting;XUE Ximei;ZHANG Qiufang(School of Oceanology and Food Science,Quanzhou Normal University,Quanzhou Fujian 362000,China;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou Fujian 350007,China;Wuyishan National Park Research Monitoring Center,Wuyishan Fujian 354300,China;Institute of Urban Environment,Chinese Academy of Sciences,Xiamen Fujian 361021,China)

机构地区：[1]泉州师范学院海洋与食品学院,福建泉州362000 [2]福建农林大学食品科学学院,福建福州350007 [3]武夷山国家公园科研监测中心,福建武夷山354300 [4]中国科学院城市环境研究所,福建厦门361021

出　　处：《泉州师范学院学报》2022年第5期22-30,共9页Journal of Quanzhou Normal University

基　　金：国家自然科学基金(41571252);福建省科技厅引导性项目(2020N0032);福建省大学生创新创业训练计划项目(S202010399068)

摘　　要：采用培养法连续两年对福建武夷山地区24个定位观测点土壤放线菌进行分离培养和纯化,并应用16S rRNA基因进行物种鉴定及其多样性分析.结果表明:(1)分离获得的109株放线菌可包含了5个目、6个科、10个属,10个属分别为链霉菌属(Streptomyces)、节杆菌属(Arthrobacter)、假节杆菌属(Pseudarthrobacter)、类节杆菌属(Paenarthrobacter)、北里孢菌属(Kitsatospora)、小单孢菌属(Micromonospora)、中国单胞菌属(Sinomonas)、拟诺卡氏菌属(Nocardiopsis)、类诺卡氏菌属(Nocardiodies)和野野村菌属(Nonomuraea).(2)分离获得链霉菌属菌株最多(79株),有42个种,是武夷山地区土壤可培养放线菌的优势菌属;其次是北里孢菌属(17株),有5个种.慧苑坑土壤分离获得的放线菌物种最多(9种),而麻粟样地海拔1280 m处只分离到1种.(3)黄溪洲海拔1200 m和鬼洞处土壤中放线菌群落的Simpson和Shannon指数均为最高,茶洞的Simpson和Shannon指数均最低.Soil actinomycetes were isolated,cultured and purified from 24 sites in Wuyishan area of Fujian Province for two consecutive years,species identification and diversity analysis were performed using 16 S rRNA gene.The results showed(1)A total of 109 strains of actinomycetes were obtained from the soils in Wuyishan area of Fujian Province,which could be classified into 5 orders,6 families and 10 genera respectively,including the genera of Streptomyces,Arthrobacter,Pseudarthrobacter,Paenarthrobacter,Kitsatospora,Micromonospora,Sinomonas,Nocardiopsis,Nocardiodies and Nonomuraea;(2)Streptomyces was the dominant taxon at the genus level of cultivable actinomycetes in the Wuyishan area of Fujian Province,and there were 42 species consisting of 79 strains,Kitsatospora was the second dominant taxon of the actinomycetes isolated,including 5 species;Huiyankeng had the most abundant species of actinomycetes(9 species)in the soils among the 24 sites,while only one species of actinomycetes was isolated from the site of Masu at an altitude of 1280 m;(3)The actinomycete community diversity index showed that the indexes of Simpson and Shannon at the site of Huangxizhou at an altitude of 1200 m and Guidong were the highest,while those of Chadong were the lowest.

关 键 词：土壤放线菌 纯培养 物种多样性 16S rRNA基因 武夷山区 

分 类 号：S154.383[农业科学—土壤学]