超高效液相色谱-串联质谱法检测德国小蠊体内氟虫腈及其代谢产物  

Determination of fipronil and its metabolites in Blattella germanica by ultra high performance liquid chromatography tandem mass spectrometry

作　　者：张祎玮[1] 王欣[1] 陈晨[1] 刘宇龙 庞松涛[1] 付晗[1] 雷晓岗 徐琨 李永波[1] 刘佳[1] ZHANG Yi-wei;WANG Xin;CHEN Chen;LIU Yu-long;PANG Song-tao;FU Han;LEI Xiao-gang;XU Kun;LI Yong-bo;LIU Jia(Xi’an Center for Disease Control and Prevention,Xi’an,Shaanxi 710054,China)

机构地区：[1]西安市疾病预防控制中心,陕西西安710054

出　　处：《中华卫生杀虫药械》2022年第5期389-393,共5页Chinese Journal of Hygienic Insecticides and Equipments

基　　金：西安市医学研究项目[编号:20YXYJ0011(1)]

摘　　要：目的采用超高效液相色谱-串联质谱法检测德国小蠊体内氟虫腈及其代谢产物。方法采用乙腈提取,QuChERS法进行样品前处理,超高效液相色谱-串联质谱法进行化合物检测。结果方法标准曲线相关系数均大于0.995,检出限均小于0.0093 mg/kg,精密度RSD值均小于4.34%,不同浓度下准确度平均值为85.9%~90.4%。结论采用此方法检测到了德国小蠊体内存在的氟虫腈及氟虫腈砜。Objective To detect fipronil and its metabolites in Blattella germanica by ultra high performance liquid chromatography tandem mass spectrometry.Methods The sample was extracted with acetonitrile,QuChERs method was employed for sample pretreatment,UPLC-MS/MS for compound detection.Results The correlation coefficient of the standard curve of fipronil and its three metabolites was better than 0.995,the detection limit was less than 0.0093 mg/kg,the RSD value of precision was less than 4.34%,and the average accuracy was between 85.9%and 90.4%.Conclusion Fipronil and fipronil sulfolane in Blattella germanica were detected by this method.

关 键 词：德国小蠊 QuChERS 液相色谱串联质谱 氟虫腈 

分 类 号：R184.3[医药卫生—流行病学] O657.63[医药卫生—公共卫生与预防医学]