羧丁酰壳聚糖-丝素肽/氧化魔芋可注射水凝胶的制备及性能  

Preparation and Properties of Carboxybutyryl Chitosan-Silk Peptide/Oxidized Konjac Injectable Hydrogel

作　　者：王博文 刘爽 樊李红 陈田田 孙慧譞 胡志海 易嘉琰 王有丽 WANG Bowen;LIU Shuang;FAN Lihong;CHEN Tiantian;SUN Huixuan;HU Zhihai;YI Jiayan;WANG Youli(School of Chemistry,Chemical Engineering and Life Sciences,Wuhan University of Technology,Wuhan 430070,Hubei,China;Rizhao Wuhan University of Technology Biomedicine and New Materials Research Institute,Rizhao 276800,Shandong,China)

机构地区：[1]武汉理工大学化学化工与生命科学学院,湖北武汉430070 [2]日照武汉理工大生物医药暨新材料研究院,山东日照276800

出　　处：《武汉大学学报（理学版）》2022年第6期589-595,共7页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金(51773161);湖北省重大专项(2019ABA115);日照市科技创新专项(2019CXZX1108)

摘　　要：利用丝素肽对羧丁酰壳聚糖进行接枝改性,再以氧化魔芋(OKGM)作交联剂,通过Schiff碱反应制备得到一种可注射的羧丁酰壳聚糖-丝素肽/氧化魔芋(NSGM)水凝胶。样品的红外光谱和磁共振氢谱证实了改性、接枝和交联反应的发生,扫描电镜结果表明该水凝胶具有内部连通的孔结构。性能研究结果表明,该水凝胶具有35~70 s可调节的凝胶时间,溶胀率最高可达1259%,室温下24 h后最多可维持21.1%的水分,同时具有良好的生物相容性。An injectable hydrogel was prepared through Schiff base reaction using silk fibroin peptide(SFP)grafted carboxybutyryl chitosan(NSC)as substrate and oxidized konjac glucomannan(OKGM)as cross linker.Successful modification,grafting and crosslinking reactions with the hydrogel material were confirmed by Fourier transform infrared(FT-IR)and~1H NMR spectrum.Porous structure of the prepared hydrogel was also verified by SEM images.Afterwards,physicochemical properties of NSGM hydrogel were investigated and results showed that the prepared hydrogel possesses an adjustable gel time varied from 35 s to 70 s.Furthermore,the maximum water absorption capability of NSGM reached as high as 1259%,and maximum 21.1%water could be retained within the hydrogel even after 24 hours at room temperature.Cytotoxicity study was then conducted to prove the superior biocompatibility of the hydrogel.

关 键 词：羧丁酰壳聚糖 丝素肽 氧化魔芋 可注射水凝胶 制备 生物活性 

分 类 号：TQ427.26[化学工程] R318.08[医药卫生—生物医学工程]