髓源性抑制细胞在慢性骨髓炎中的作用研究  

The role of myeloid-derived suppressor cells in chronic osteomyelitis

作　　者：冯建波 杨礼丹 程飘涛 李陈诚 刘金月 彭笳宸 FENG Jianbo;YANG Lidan;CHENG Piaotao;LI Chencheng;LIU Jinyue;PENG Jiachen(Department of Orthopedics,Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China;Department of Joint Surgery,People’s Hospital of Zheng’an,Zunyi 563400,China;Joint Orthopaedic Research Center of Zunyi Medical University&University of Rochester Medical Center,Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医科大学附属医院骨科,563000 [2]正安县人民医院关节外科,遵义563400 [3]遵义医科大学-罗切斯特大学联合骨科研究中心,遵义563000

出　　处：《免疫学杂志》2023年第10期893-899,共7页Immunological Journal

基　　金：国家自然科学基金项目(81760400);贵州省科技计划项目(黔科合支撑[2018]2760)

摘　　要：目的研究髓系来源的抑制性细胞(myeloid-derived suppressor cells,MDSCs)在大鼠慢性骨髓炎疾病中的免疫作用,为骨髓炎的治疗探索新方法。方法首先建立大鼠慢性骨髓炎模型,并使用吉西他滨抑制MDSCs增长,通过流式细胞术及免疫荧光检测大鼠骨髓及脾脏中的MDSCs比例,ELISA检测外周血中炎性因子变化,分析正常大鼠、骨髓炎大鼠以及吉西他滨抑制后的大鼠外周血中炎性因子水平(TNF-α、PCT、IL-4、IL-10、IL-17、IFN-γ、TGF-β)与骨髓中MDSCs的相关性,分析MDSCs在慢性骨髓炎疾病中的免疫作用。结果骨髓炎模型大鼠骨髓及脾脏中的MDSCs细胞比例增高,使用吉西他滨组MDSCs细胞比例明显降低(P<0.05),炎性因子水平(TNF-α、PCT、IL-4、IL-10、IL-17、IFN-γ、TGF-β)与MDSCs的细胞比例变化呈正相关(P<0.05)。结论大鼠骨髓炎中MDSCs细胞比例变化与炎性因子水平呈正相关,吉西他滨抑制MDSCs可能通过抑制MDSCs而降低炎性反应,促进机体修复。Inhibitory cells derived from bone marrow are a kind of inhibitory cells derived from bone marrow.These cells are not only related to tumor growth,but also participate in the inflammatory immune process.Therefore,we established a rat model of chronic osteomyelitis,and used gemcitabine to inhibit the cell growth ratio of MDSCs.We detected the ratio of MDSCs in bone marrow and spleen of rats by flow cytometry and immunofluorescence,detected the changes of inflammatory factors in peripheral blood by ELISA,and analyzed the inflammatory factors(TNF-α,PCT,IL-4,IL-10,IL-11)in peripheral blood of normal rats,osteomyelitis rats and rats after gemcitabine inhibition.The results showed that the proportion of MDSCs cells in bone marrow and spleen of osteomyelitis model rats was increased,but it was significantly decreased in gemcitabine group(P<0.05).Levels of inflammatory factors(TNF-α,PCT,IL-4,IL-10,IL-17,IFN-γ,TGF-β)were positively correlated with the change of MDSCs cell proportion(P<0.05).From the results,it can be inferred that the change of the proportion of MDSCs cells in rat osteomyelitis is positively related to the inflammatory factors,and gemcitabine can reduce inflammatory factors by inhibiting MDSCs.

关 键 词：髓源性抑制细胞 骨髓炎 金黄色葡萄球菌 细菌生物膜 

分 类 号：R392-33[医药卫生—免疫学]