基于UPLC-Q-TOF-MS/MS代谢组学技术研究炮制前后何首乌化学成分变化  

Changes of Chemical Components of Crude and Processed Heshouwu(Polygoni Multiflori Radix)Products Using Ultra-Performance Liquid Chromatography-Quadrupole/Time-of-Flight Mass Spectrometry Based on Metabolomic Approach

作　　者：王敏[1] 王晶[2] 杨娜[1] 柳航[1] 葛卫红[1] WANG Min;WANG Jing;YANG Na;LIU Hang;GE Weihong(The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008,Jiangsu,China;School of Pharmacy,Shaanxi University of Traditional Chinese Medicine,Shaanxi 712046,Xianyang,China)

机构地区：[1]南京大学医学院附属鼓楼医院药学部,江苏南京210008 [2]陕西中医药大学药学院,陕西咸阳712046

出　　处：《辽宁中医杂志》2022年第2期149-153,224-225,共7页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金(81903892);江苏省自然科学基金(BK20180129);江苏省药学会-奥赛康医院药学科研项目(A201906)

摘　　要：目的利用代谢组学分析方法对比分析市售生首乌和制首乌化学成分的差异。方法采用超高效液相色谱-四级杆飞行时间质谱(UPLC-Q-TOF-MS/MS)方法,通过主成分分析和偏最小二乘判别分析等方法对比分析市售生首乌和制首乌化学成分的差异。结果市售生首乌和制首乌化学成分差异较大,共鉴别45个差异化学成分,主要为蒽醌、二苯乙烯和鞣质等。结论基于UPLC-Q-TOF-MS/MS代谢组学技术从整体角度分析了市售生首乌和制首乌的化学成分差异,为其炮制机理的阐释和临床应用奠定基础。Objective To explore the chemical components changes between crude Heshouwu(Polygoni Multiflori Radix,PMR)(C-PMR)and processed Heshouwu(Polygoni Multiflori Radix)(P-PMR)products in the market.Methods An ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)based metabolomic approach was adopted.Then the acquired data was processed by principal component analysis and partial least squares-discriminant analysis to reveal their chemical differences.Results There was significant difference of the chemical components changes between C-PMR and P-PMR.A total of forty-five differential compounds,including anthraquinones,stilbenes,tannins and so on,were identified.Conclusion The UPLC-Q-TOF-MS/MS based metabolomic approach analyzed the chemical differences between C-PMR and P-PMR products in a holistic view,which would provide a theoretical basis for the mechanism interpretation and clinical application of different PMR products.

关 键 词：何首乌 炮制 超高效液相色谱-四级杆飞行时间质谱 代谢组学 

分 类 号：R284.1[医药卫生—中药学]