应用分子标记快速检测楚玉2号萝卜新品种对根肿病的抗性研究  

Study on rapid detection of resistance of new radish variety Chuyu No.2 to clubroot by molecular marker

作　　者：杨谦 崔磊[1] 严承欢 潘新春 任志勇 於校青 王冲 邓晓辉[1] 甘彩霞[1] YANG Qian;CUI Lei;YAN Cheng-huan;PAN Xin-chun;REN Zhi-yong;YU Xiao-qing;WANG Chong;DENG Xiao-hui;GAN Cai-xia(Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement/Institute of Economic Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Wuhan Pindao Architectural Garden Engineering Co.,Ltd.,Wuhan 430000,China;Wuhan Dongxihu District Agricultural and Rural Bureau,Wuhan 430040,China)

机构地区：[1]蔬菜种质创新与遗传改良湖北省重点实验室/湖北省农业科学院经济作物研究所,武汉430064 [2]武汉品道建筑园林工程有限公司,武汉430000 [3]武汉市东西湖区农业农村局,武汉430040

出　　处：《湖北农业科学》2022年第S01期225-228,共4页Hubei Agricultural Sciences

基　　金：国家重点研发计划项目(2021YFD1600303-2);湖北省农业科技创新中心创新团队项目(2021-620-000-001-007)

摘　　要：运用3个分子标记对从50 kg楚玉2号萝卜(Raphanus sativus L.)种子随机取样的64粒种子进行了根肿病抗性的分子标记检测,同时开展了室内接种鉴定。结果表明,64个样本均含有3个分子标记的特异抗病条带,显示具有抗病性,与室内接种鉴定结果一致,说明该批种子具有根肿菌的3个抗性位点。Three molecular markers were used to detect the clubroot resistance locus of 64 plants randomly selected from 50 kg Chuyu No.2 radish seeds by molecular marker detection,at the same time indoor inoculation identification was also carried out.The results showed that 64 plants all contained specific resistance bands with three molecular markers,showing resistance,which was consistent with the results of indoor inoculation identification,indicating that the batch of seeds had 3 resistance sites of clubroot.

关 键 词：萝卜(Raphanus sativus L.) 种子 根肿病 分子标记 检测 

分 类 号：S631.1[农业科学—蔬菜学]