基于二硫化钼负载双金属电化学免疫传感器同时检测2种心肌肌钙蛋白抗原  

Simultaneous detection of 2 cardiac troponin antigens based on MoS_(2)-loaded bimetallic electrochemical immunosensor

作　　者：姜丽萍 尹起 韩洪文[1] 孙静波[1] 张洪静 JIANG Liping;YIN Qi;HAN Hongwen;SUN Jingbo;ZHANG Hongjing(College of Engineering,Yantai Nanshan University,Yantai 265713,China;Hangxin Material Technology Co.,Ltd.,Yantai 264006,China)

机构地区：[1]烟台南山学院工学院,烟台265713 [2]航鑫材料科技有限公司,烟台264006

出　　处：《分析试验室》2023年第7期883-890,共8页Chinese Journal of Analysis Laboratory

基　　金：山东省自然科学基金(ZR2019PB010);龙口市科技研发计划(2020KJJH061);南山集团科技计划项目(2022HX004)资助

摘　　要：心肌肌钙蛋白I蛋白(cTnI)和心肌肌钙蛋白T蛋白(cTnT)是诊断急性心肌梗死的关键生物标志物。以电沉积金作为传感平台,用硫堇(Thi)和二茂铁(Fc)功能化二硫化钼负载双金属(MoS_(2)-BMNPs)作为检测抗体标记物制备电化学免疫传感器,用于同时检测cTnI和cTnT。结果表明:MoS_(2)-BMNPs具有良好的导电性和双金属协同效应,可放大检测信号,提高检测灵敏度。通过方波伏安法(SWV)检测到-0.3 V和0.3 V处出现2个尖锐信号峰,峰值位置和电流强度反映相应抗原的浓度。该免疫传感器可同时定量检测cTnI和cTnT,线性范围均为0.01~100 ng/mL,检出限分别为3.67 pg/mL和3.33 pg/mL。Cardiac troponin I protein(cTnI)and cardiac troponin T protein(cTnT)are key biomarkers for the diagnosis of acute myocardial infarction(AMI).In this work,electro-deposited gold was used as a sensing platform.MoS_(2)-hybridized bimetallic nanoparticles(MoS_(2)-BMNPs)functionalized by thionine(Thi)and ferrocene(Fc)were used as markers tofabricate electrochemical immunosensor for the simultaneous determination of cTnI and cTnT.The results showed that MoS_(2)-BMNPs with excellent electrical conductivity and bimetallic synergistic effect could enhance the square wave voltammetry(SWV)signal to improve the detection sensitivity.The SWV method was used to record two sharp signal peaks at-0.3 V and 0.3 V.The peak position and current intensity revealed the concentration of the corresponding antigens.The immunosensor could be used for simultaneous quantitative detection of cTnI and cTnT with linear ranges of 0.01-100 ng/mL,and the detection limits were 3.67 pg/mL and 3.33 pg/mL,respectively.

关 键 词：二硫化钼负载双金属 心肌肌钙蛋白 同时检测 电化学免疫传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]