糖尿病肾病生物标志物电化学检测方法研究进展  

Recent Advances in Electrochemistry Assays of Diabetic Kidney Disease Biomarkers

作　　者：田野 高丽丽 张巍[3,4] 娄保华[3] 关怡然[3] 徐国宝[3,4] TIAN Ye;GAO Li-Li;ZHANG Wei;LOU Bao-Hua;GUAN Yi-Ran;XU Guo-Bao(The College of Civil Engineering,Shenyang Urban Construction University,Shenyang 110167,China;School of Materials Science and Engineering,Shenyang Jianzhu University,Shenyang 110168,China;State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]沈阳城市建设学院土木工程学院,沈阳110167 [2]沈阳建筑大学材料科学与工程学院,沈阳110168 [3]中国科学院长春应用化学研究所,电分析化学国家重点实验室,长春130022 [4]中国科学技术大学应用化学与工程学院,合肥230026

出　　处：《分析化学》2023年第5期744-756,共13页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.22174136,22004116)资助

摘　　要：糖尿病肾病(Diabetic kidney disease,DKD)是由糖尿病引起的肾小球和肾小管微血管病变,是糖尿病最常见和最严重的慢性并发症之一。该病情早期具有一定的隐匿性,临床症状出现较晚,而一旦发展到终末期肾脏病,通常比其它肾脏疾病的治疗难度更大。因此,检测DKD的早期生物标志物对减少肾功能损伤具有重要意义。但是,目前尚无某个单独指标可以对DKD进行早期诊断,临床上多采用多种指标结合症状进行诊断。因此,发展简单、高效、灵敏的多重标志物检测方法对于DKD的早期诊断显得尤为重要。本文以microRNAs、胱抑素C、同型半胱氨酸、肌酐、Ⅳ型胶原蛋白和N-乙酰氨基葡萄糖苷酶等常见的DKD标志物为例,对近几年发展的DKD标志物检测方法进行了评述,着重介绍了电化学分析法(Electrochemistry,EC)尤其是电化学发光分析法(Electrogenerated chemiluminescence,ECL)在DKD标志物检测中的应用进展,并对其未来的发展方向进行了展望。Diabetic kidney disease(DKD)is the microvascular disease of the glomeruli and renal tubules as a result of diabetes.It is one of the most frequent and serious chronic complications of diabetes.The early stage of DKD has a certain occult and the clinical symptoms appear late.It has a certain concealment in the early stage and it is often more difficult to treat than other renal diseases.Therefore,the early detection of DKD biomarker is of great significance to reduce renal damage.However,there is no single indicator for early diagnosis of DKD.The combination of multiple indicators and symptoms are used in its clinical diagnosis.Hence,the establishment of simple,efficient and sensitive multi-biomarker detection platforms for the early diagnosis of DKD is much demanded.In this review,taking different types of DKD biomarkers(microRNAs,cystatin C,homocysteine,creatinine,type IV collagen,and n-acetylglucosaminidase)as examples,a comprehensive summary of the recent developments of various types of electrochemical detection platforms was presented for them over recent years,such as electrochemistry(EC)and electrogenerated chemiluminescence(ECL),and its further development was prospected.

关 键 词：糖尿病肾病 生物标志物 电化学 电化学发光 评述 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学] O657.1[医药卫生—临床医学]