氧化应激对子宫内膜异位症患者体外受精-胚胎移植妊娠结局的影响  

Impact of oxidative stress on the pregnancy outcome of in vitro fertilization-embryo transfer in patients with endometriosis

作　　者：韩彦洁[1] 蒋彦[2] 杨倩[3] 樊涛[4] 张瑶[1] 许红[1] HAN Yanjie;JIANG Yan;YANG Qian;FAN Tao;ZHANG Yao;XU Hong(Department of Blood Transfusion,The Fourth Hospital of Shijiazhuang,Shijiazhuang 050011,China)

机构地区：[1]石家庄市第四医院输血科,050011 [2]石家庄市第四医院生殖中心,050011 [3]石家庄市第四医院病理科,050011 [4]石家庄市第四医院新生儿科,050011

出　　处：《中国妇产科临床杂志》2020年第2期138-140,共3页Chinese Journal of Clinical Obstetrics and Gynecology

摘　　要：目的探讨氧化应激对子宫内膜异位症(EMs)患者体外受精-胚胎移植(IVF-ET)妊娠结局的影响。方法收集EMs合并不孕患者65例为EMs组,输卵管因素引起不孕患者165例作为对照组,分别比较两组患者一般资料、促排卵指标、实验室指标、外周血和卵泡液中氧化应激指标。结果EMs组和对照组的促性腺激素Gn使用时间、基础卵泡刺激素(bFSH)、黄体生成素(LH)、抗苗勒管激素(AMH)比较,差异均无统计学意义(P>0.05),而EMs组患者Gn使用量显著增加(P<0.05)。EMs组患者的平均获卵数、受精率、优质胚胎率、可用胚胎率、临床妊娠率、流产率分别为(9.12±3.50)枚、(50.96±7.01)%、(20.71±2.21)%、(40.33±6.12)%、(40.98±5.34)%和(20.00±3.39)%,对照组分别为(18.54±3.92)枚、(70.83±8.23)%、(30.13±4.47)%、(56.64±7.87)%、(54.42±2.98)%和(7.14±2.86)%,两组比较,差异均有统计学意义(P<0.05);EMs患者外周血中氧化物歧化酶(superoxide dismutase,SOD)及氧化应激指标活性氧(reactive oxygen species,ROS)表达量分别为(12.60±0.59)U/L和63.67±2.31,卵泡液中分别为(6.23±0.31)U/L和70.00±2.00,对照组外周血和卵泡液分别为(19.40±0.53)U/L、43.53±1.36、(10.2±0.60)U/L和50.33±1.53,与对照组比较,外周血和卵泡液中ROS显著升高,SOD显著降低(P<0.01)。结论EMs合并不孕患者ROS和SOD异常引起的氧化应激紊乱可能与其IVF-ET妊娠结局不良有关。Objective To investigate the factor that influences the pregnancy outcome of in vitro fertilization-embryo transfer(IVF-ET)in patients with endometriosis(EMs).Methods A total of 65 EMs patients combined infertility(EMs group)and 165 infertility patients caused by fallopian tubal factors(control group)were enrolled.General information,ovulation induction indicators,laboratory indicators,and oxidative stress indicators in peripheral blood and follicular fluid were compared between the two groups.Results The usage time of Gn,the levels of basal follicle-stimulating hormone(bFSH),luteinizing hormone(LH),anti-muller-tube hormone(AMH)showed no significant difference between two groups(P>0.05),butthe usage amount of Gn in the EMs was significantly increased(P<0.05).The average number of eggs,fertility rate,high quality of embryo rate,available embryo rate,clinical pregnancy rate and abortion rate were(9.12±3.50),(50.96±7.01)%,(20.71±2.21)%,(40.33±6.12)%,(40.98±5.34)%,(20.00±3.39)%in EMs group,and(18.54±3.92),(70.83±8.23)%,(30.13±4.47)%,(56.64±7.87)%,(54.42±2.98)%,(7.14±2.86)%in control group.There were significant differences between the two groups(P<0.05).The expression levels of superoxide dismutase(SOD)and reactive oxygen species(ROS)in peripheral blood of EMs patients were(12.60±0.59)U/L and 63.67±2.31,respectively,and(6.23±0.31)U/L and 70.00±2.00 in follicular fluid.The control group were(19.40±0.53)U/L,43.53±1.36,(10.2±0.60)U/L,and 50.33±1.53,respectively.Compared with the control group,ROS in the peripheral blood and follicular fluid were significantly increased and SOD was significantly decreased in EMs group(P<0.01).Conclusion The oxidative stress disorder caused by abnormal ROS and SOD in infertility patients combined with EMs may be related to the adverse pregnancy outcome of IVF-ET.

关 键 词：子宫内膜异位症 体外受精-胚胎移植 氧化应激水平 妊娠结局 

分 类 号：R714.8[医药卫生—妇产科学]