基于发光二极管对的微型荧光光度计的研究  

Study on Micro Fluorophotometer System based on Paired Emitter-detector Diode

作　　者：黄利强[1,2] HUANG Li-qiang(Fisheries College,Jimei University,Xiamen 361021,China;Water environment and fishery resources monitoring center,Jimei University,Xiamen 361021,China)

机构地区：[1]集美大学水产学院,福建厦门361021 [2]集美大学水域环境与渔业资源监测中心,福建厦门361021

出　　处：《化学工程与装备》2020年第12期257-259,58,共4页Chemical Engineering & Equipment

摘　　要：本文设计和制作了一款以发光二极管对为光源和检测器的低成本荧光光度计系统,具有结构简单、成本低廉、迷你便携、操作简单的特点。系统使用660nm/703nm的发光二极管对为光源/检测器,用数字电压表直接显示样品的荧光强度值。使用自制的荧光光度计对亚甲基蓝试样进行了测试,测定结果与商用荧光分光光度计较为相近,具有较好的线性度和精密度,有望投入实际应用。A low-cost micro-fluorophotometer system with pair light-emitting diode as light source and detector is designed and manufactured.The system has the advantages of simple structure,low cost,portable and easy to use.The system uses 660 nm/703 nm LEDs as light source/detector and a digital voltmeter to display fluorescence intensity directly.Methylene blue samples were tested by self-made fluorophotometer and results were similar to commercial spectrophotometer.The system has good linearity and precision,and is expected to be put into practical application.

关 键 词：荧光光度计 发光二极管对 亚甲基蓝 

分 类 号：TH744.1[机械工程—光学工程] TN312.8[机械工程—仪器科学与技术]