长穗鱼尾葵花粉主要变应原的研究  

Research on the total allergens of carvotamitis pollen

作　　者：谢伟伟 刘硕 孟光 龙绮[1] 陈菊蓉 XIE Wei-wei;LIU Shuo;MENG Guang;LONG Qi;CHEN Ju-rong(Department of Otolaryngology&Head and Neck Surgery,Central South University Xiangya School of Medicine Affiliated Haikou Hospital,Haikou 570208,Hainan Province,China)

机构地区：[1]中南大学湘雅医学院附属海口医院耳鼻咽喉头颈外科,海南海口570208

出　　处：《中国临床药理学杂志》2020年第3期363-365,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81160129);海南省自然科学基金资助项目(30871,811145);海南省国际科技合作重点计划基金资助项目(2012-GH010);海口市重点科技计划基金资助项目(海科工信立[2012]72号);海口市医学特色专科-海卫字[2015]15号(耳鼻喉专业).

摘　　要：目的探讨用双向电泳对长穗鱼尾葵花粉提取液蛋白质组分进行分析的价值,并用蛋白质印迹法寻找鱼尾葵花粉主要变应原。方法提取长穗鱼尾葵花粉变应原,用双向电泳(2-DE)结合蛋白质印迹法,用长穗鱼尾葵花粉过敏阳性患者和健康对照者分别作为阳性和阴性组,获得相应的电泳图谱和免疫印迹图,对比结果以获得特异性变应原蛋白点。结果长穗鱼尾葵花粉蛋白提取液经双向电泳后,考马斯亮蓝染色,在分子量为10~170 k Da和等电点p I3.0~10.0发现约604个蛋白质点。蛋白质印迹法结果,阳性和阴性组对比,特异性变应原蛋白点数约为3个。结论可以用双向电泳联合蛋白质印迹法对长穗鱼尾葵花粉提取液蛋白质组分分析,并可进一步用于变应原致敏组分的研究。Objective phoresis(2-DE)in protein analysis of allergen extract and to find the main allergens of carvotamitis pollen.Methods Carvotamitis pollen extract was prepared with routine method,two dimentional electrophoresis(2-DE)combined with immunoblotting assay(western-blotting).Patients with positive allergy to Carvotamitis pollen and healthy controls were used as the positive and negative groups,to obtain the 2-DE electrophoretogram and Western blotting map.Then the positive and negative results were compared to obtain specific protein spots.Results 604 soluble proteins spots were revealed in coomassie-stained gels.Most of the proteins had a molecular weight(Mr)of 10-170 k Da,and an isoelectric point(pI)value of 3.0 to 10.0.Immunoblotting of2-DE were shown and there were 3 specific antigen spots,compared with the controls.Conclusion(2-DE)is a good method in protein analysis of carvotamitis pollen allergen extract,its potential value in allergic components needs to be further studied.

关 键 词：长穗鱼尾葵花粉 变应原 双向电泳 蛋白质印迹法 

分 类 号：R446.6[医药卫生—诊断学]