Molecular detection of oxacillinase genes and typing of clinical isolates of Acinetobacter baumannii in Tehran, Iran  

作　　者：Mostafa Alavi-Moghaddam Mehran Dolati Abdolreza Javadi Bahareh Ghobadi Saki Morteza Karami-Zarandi Saeed Khoshnood 

机构地区：[1]Emergency Medicine Departent,Shahid Beheshti University of Medical Sciences,Tehran,Iran [2]Clinical Research Development Unit,Imam Hossein Hospital,Shahid Beheshti University of Medical Sciences,Tehran,Iran [3]Department of Microbiology,School of Medicine,Tehran University of Medical Sciences,Tehran,Iran [4]Student Research Committee,School of Medicine,Ahvaz Jundishapur University of Medical Sciences,Ahvaz,Iran

出　　处：《Journal of Acute Disease》2020年第1期33-39,共7页急性病杂志（英文版）

摘　　要：Objective:To determine patterns of antimicrobial resistance,analyze the prevalence of oxacillinase and molecular typing of strains of Acinetobacter baumannii(A.baumannii).Methods:A total of 121 strains of A.baumannii were obtained from patients admitted to Imam Hossein and Imam Khomeini Hospitals,Tehran,Iran,from January 2016 to November 2018.Antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion method according to the Clinical and Laboratory Standards Institute recommendations.The presence of oxacillinase genes was assessed by polymerase chain reaction(PCR).To determine clonal relatedness,all isolates were subjected to repetitive sequence-based PCR(REP-PCR).Results:The isolates were obtained from 56(46.3%)males and 65(53.7%)females with the mean age of 39.5 years.Colistin with 100.0%sensitivity rate had the highest effect,while ceftriaxone with 16.5%sensitivity rate had the least effect on A.baumannii isolates.In addition,96(79.3%)and 99(81.8%)isolates were resistant to imipenem and meropenem,respectively.A total of 109 isolates(90.0%)exhibited multiple drug resistance with 10 different resistotypes.In total,75(75.7%)of carbapenem resistant isolates were positive for blaOXA-23-like,and 14(14.1%)for blaOXA-24-like gene.The five main clones A,B,C,D,and E were detected in 25(25.2%),36(36.4%),10(10.1%),8(8.0%),and 6(6.1%)of isolates,respectively.Conclusions:Carbapenem-resistant A.baumannii strains are high in the current study.To control the spread of carbapenem-resistant A.baumannii strains,regular monitoring programs are needed.

关 键 词：ACINETOBACTER BAUMANNII MULTIDRUG resistance Oxacillinase REPETITIVE sequence-based PCR 

分 类 号：R37[医药卫生—病原生物学]