HPLC法测定复方丹参丸中丹酚酸B的含量  

Determination of Salvianolic acid B in Fufang Danshen Pill by HPLC

作　　者：张倩[1] 孙艳丽[1] 高晓艳[1] 陈美玲[1] 凌娅[1] ZHANG Qian;SUN Yan-lii;GAO Xiao-yan;CHEN Mei-ling;LING Ya(Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222047,Jiangsu,China)

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222047

出　　处：《医学信息（医学与计算机应用）》2016年第36期115-116,共2页Medical Information

摘　　要：目的:建立复方丹参丸中丹酚酸B含量测定方法。方法采用高效液相色谱法,Kromasil C18色谱柱((250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸(22:78),检测波长为286nm;柱温30℃;流速1.0ml·min-1。结果丹酚酸B在0.1034~1.034μg范围内,呈良好的线性关系(r=1.000),回收率为98.2%,RSD为1.2%(n=9)。结论本方法简便、准确。方法的稳定性、重复性良好,可作为复方丹参丸的质量控制。Objective To establish a HPLC method for Salvianolic acid B in Fufang Danshen Pill.Methods A Kromasil C18(250mm×4.6mm,5μm)column was used with the mobile phase was acetonitrile-0.1%phosphoric acid(22:78),at the detection wavelength was 286nm.The column temperature was 30℃.The flow rate was 1.0ml·min-1.Results Salvianolic acid B was linear at the range of 0.1034μg^1.034μg(r=1.000).The recovery was 98.2%,and RSD was1.2%(n=9).Conclusion The method is specific and accurate.The method are stable with good reproducibility,and can be used for the quality control of Fufang Danshen Pill.

关 键 词：复方丹参丸 丹酚酸B 高效液相色谱 

分 类 号：R28[医药卫生—中药学]