姜黄素对带状疱疹后神经痛大鼠脊髓背角及背根神经节p-CREB表达的影响  

Influence of curcumin on expression of cAMP response element-binding protein phosphorylation (p-CREB) in spinal dorsal horn and dorsal root ganglion in a rat model of herpes zoster postherpetic neuralgia

作　　者：皮治兵[1] 林海[1] 蔡振 彭宇[1] 张钧凯[1] 金元[1] Pi Zhibing;Lin Hai;Cai Zhen;Pen Yu;Zhang Junkai;Jin Yuan(Department of Pain Medicne,First Affiliated Hospital of Wenzhou Medical University,Zhejiang Province 325000,China)

机构地区：[1]温州医科大学附属第一医院疼痛科,325000 [2]金华市中心医院麻醉科

出　　处：《实用疼痛学杂志》2018年第4期251-256,共6页Pain Clinic Journal

基　　金：2011年浙江省医药卫生平台骨干人才计划(2011RCB026).

摘　　要：目的 观察姜黄素对带状疱疹后神经痛大鼠脊髓背角及背根神经节p-CREB表达的影响.方法 雄性SD大鼠80只,采用随机数字表法,将其分为4组(n=20):对照组(C组),模型组(D组),姜黄素1组(DCur1),姜黄素2组(DCur2).对照组(C组)在后爪注射0.9%氯化钠溶液;模型组(D组)在后爪注射人乳头瘤病毒-1(HSV-1,浓度为106/100μl,注射容积50μl)制备疱疹后神经痛动物模型;DCur1组和DCur2组于造模5d后,分别腹腔内注射姜黄素100、200 mg/kg,1次/d,持续7d.于造模前、造模后5d(治疗前)及治疗结束后1、7、14d,测定大鼠机械缩足阈值、热缩足潜伏期;各组于治疗结束后14d时处死8只大鼠,检测脊髓背角和背根神经节(DRG)中磷酸化cAMP反应元件结合蛋白(p-CREB)的表达.结果 与C组比较,其他3组造模后5d及治疗后1、7d时MWT值均下降,TWL值均缩短(P<0.05);与D组比较,治疗后7、14d时,DCur1组和DCur2组MWT值均升高,TWL值均延长,DCur2组变化更明显(P>0.05).与C组比较,其他3组大鼠脊髓背角区和背根神经节p-CREB表达均升高(P<0.05);与D组比较,DCur1组和DCur2组p-CREB表达均下调,且DCur2组下调更明显(P<0.05).结论 姜黄素可缓解带状疱疹后神经痛,其机制与脊髓背角和背根神经节p-CREB过度表达下调有关.Objective To evaluate the influence of curcumin on expression of phosphorylated cAMP response element-binding protein(p-CREB)in spinal dorsal horn and dorsal root ganglion in a rat model of herpes zoster postherpetic neuralgia.Methods Eighty male SD rats were randomly divided into 4 groups(n=20):control group(group C),model group(group D),curcumin 1 group(DCur1)and curcumin 2 group(DCur2).Rats received injection of 0.9%sodium chloride solution in posterior claw in group C,received injection of human papillomavirus-1(HSV-1)(concentration of 106/100μl,injection volume 50μl)on the hind paws of rats in group D.Intraperitoneal curcumin(100,200 mg/kg,once a day for 7 days)was injected respectively at 5 days after establishment of the model in group DCur1 and group DCur2.Mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were tested after 5 d(before treatment)and 1,7,14 d after the treatment.Eight rats were sacrificed on 14 days after the treatment,and the expression of p-CREB was detected in the dorsal horn of the spinal cord and the dorsal root ganglion(DRG)in all groups.Results Compared with group C,MWT was significantly decreased,TWL was significantly shortened at each time point in the other three groups(P<0.05).Compared with group D,MWT was significantly increased,TWL was significantly extended at 7,14 d after the treatment in group DCur1 and group DCur2,especially in group DCur2(P<0.05).Compared with group C,the expression of p-CREB was up-regulated in spinal dorsal horn and dorsal root ganglion in the other three groups(P<0.05).Compared with group D,the expression of p-CREB was down-regulated in group DCur1 and group DCur2,especially in group DCur2(P<0.05).Conclusion Curcumin can alleviate herpes zoster postherpetic neuralgia in rats,and down-regulation of p-CREB over-expression in spinal dorsal horn and dorsal root ganglion is involved in the mechanism.

关 键 词：姜黄素 神经痛 带状疱疹后 CREB结合蛋白 

分 类 号：R28[医药卫生—中药学]