Distribution of extended-spectrum β-lactamase genes in antibiotic-resistant strains of Pseudomonas aeruginosa obtained from burn patients in Ahvaz, Iran  

作　　者：Saeed Khoshnood Azar Dokht Khosravi Nabi Jomehzadeh Effat Abbasi Montazeri Moloudsadat Motahar Fatemeh Shahi Morteza Saki Sakineh Seyed-Mohammadi 

机构地区：[1]Infectious and Tropical Diseases Research Center,Health Research Institute,Ahvaz Jundishapur University of Medical Sciences,Ahvaz,Iran [2]Student Research Committee,Ahvaz Jundishapur University of Medical Sciences,Ahvaz,Iran [3]Department of Microbiology,Faculty of Medicine,Ahvaz Jundishapur University of Medical Sciences,Ahvaz,Iran

出　　处：《Journal of Acute Disease》2019年第2期53-57,共5页急性病杂志（英文版）

摘　　要：Objective: To evaluate the drug susceptibility profiles and the frequency of beta-lactamase encoding genes in Pseudomonas aeruginosa (P. aeruginosa) obtained from burn patients. Methods: Totally 93 non-duplicate clinical isolates of P. aeruginosa were recovered from burn patients of Taleghani Burn Hospital of Ahvaz. Antibiotic susceptibility testing was conducted by disk diffusion method according to the CLSI 2017 recommendations. PCR assay was performed by to find beta-lactamase encoding genes. Results: In this study, most clinical specimen was obtained via wound swabs [65 (69.9%)], followed by blood [14 (15.1%)] and biopsy (7 (7.5%))Forty-two (45.16%) patients were male and 51(54.84%) were female. High resistance was observed for most of antibiotics especially for gentamicin and ciprofloxacin (Up to 85%), whereas the highest susceptibility was reported for colistin (100.0%), followed by ceftazidime (66.7%). According to PCR results, 16.1% (15), 9.7% (9) and 14.0% (13) of isolates carried blaDHA, blaVEB and blaGES genes, respectively. It also revealed that the blaVEB gene was found to coexist within 2 isolates (2.2%). Conclusions: Antibacterial resistance is high among P. aeruginosa isolates. Colistin is highly active against multi-drug resistant P. aeruginosa isolates. Antimicrobial susceptibility testing can confine indiscriminate uses of antibiotics and resistance increase, and can improve management of treatment.

关 键 词：PSEUDOMONAS AERUGINOSA BURN patients Drug sensitivity Extended-spectrum BETA-LACTAMASE 

分 类 号：R5[医药卫生—内科学]