HPLC法测定补元合肾酒中人参皂苷Rg1、Re、Rb1的含量  

Study on Quality Standard of Buyuanheshen Vinum

作　　者：卢瀚思 辜勇军 黄娟[2,3] 龚琳慧[1] 白俊毅 

机构地区：[1]四川大学生物治疗国家重点实验室,四川成都610041 [2]成都肛肠专科医院,四川成都610041 [3]成都中医药大学,四川成都610041

出　　处：《今日健康》2016年第10期344-345,共2页

基　　金：四川省科技厅项目:黄济川经典秘方'济川养生驻颜不老方'药酒的开发研究,项目(2014JY0188).

摘　　要：目的:建立补元合肾酒中人参皂苷Rg1、Re、Rb1的含量测定方法.方法:采用高效液相法对制剂中的人参皂苷Rg1、Re、Rb1进行含量测定.结果:人参皂苷Rg1、Re、Rb1进样量分别在39.16~1174.8ng、18.774~563.22ng、36.768~1103.04ng范围内与峰面积呈现良好的线性关系(r>0.999);平均回收率分别为99.84%,99.31%,99.76%,RSD为1.08%,1.96%,1.37%.结论:样品处理方法合理,方法学考察符合定量要求,结果准确,可用于补元合肾酒中人参皂苷Rg1、Re、Rb1的含量检测.Objective:To establish a method for the determination of ginsenoside Rg 1,Re,Rb1 in Buyuanheshen Vinum.Methods:The contents of ginsenoside Rg1,Re Rb1 in Buyuanheshen Vinum were determined by HPLC.Results:The content of ginsenoside Rg1,Re,Rb1 showed good liner relationships with respective peak area within the range of 39.16~1174.8ng,18.774~563.22ng and 36.768~1103.04ng(r>0.999),respectively;and the average recovery were 99.84%,99.31%and 99.76%,RSD were 1.08%,1.96%,1.37%.Conclusion The quantitative method was simple,specific,accurate and reliable,which has good reproducibility and the ability of effectively controling the quality of compound Buyuanheshen Vinum.

关 键 词：补元合肾酒 人参皂苷RG1 人参皂苷RE 人参皂苷RB1 HPLC 

分 类 号：R28[医药卫生—中药学]