Zoonotic human liver flukes,a type 1 biocarcinogen,in freshwater fishes:genetic analysis and confirmation of molluscan vectors and reservoir hosts in Bangladesh  

作　　者：Sharmin Shahid Labony Md.Abdul Alim Muhammad Mehedi Hasan Md.Shahadat Hossain Sharmin Akter Joydeep Paul Thahsin Farjana Md.Haydar Ali Mohammad Zahangir Alam Takeshi Hatta Hayato Kawada Keiko Mizutani Naotoshi Tsuji null Anisuzzaman 

机构地区：[1]Department of Parasitology,Faculty of Veterinary Science,Bangladesh Agricultural University,Mymensingh 2202,Bangladesh [2]Department of Fisheries Technology,Faculty of Fisheries,Bangladesh Agricultural University,Mymensingh 2202,Bangladesh [3]Department of Biotechnology,School of Life Sciences&Biotechnology,Adamas University,Barasat-Barrackpore Rd,Jagannathpur,Kolkata,West Bengal 700126,India [4]Department of Pathology and Parasitology,Hajee Mohammad Danesh Science and Technology University,Dinajpur 5200,Bangladesh [5]Department of Parasitology and Tropical Medicine,Kitasato University School of Medicine,1-15-1 Kitasato,Minami,Sagamihara,Kanagawa 252-0374,Japan [6]Department of Molecular and Cellular Parasitology,Kitasato University Graduate School of Medical Sciences,1-15-1 Kitasato,Minami,Sagamihara,Kanagawa 252-0373,Japan

出　　处：《Infectious Diseases of Poverty》2024年第3期73-88,共16页贫困所致传染病（英文）

基　　金：funded by the Ministry of Education(MoE)and Ministry of Science and Technology(MoST),The Government of Bangladesh;supported by the JSPS Core-to-Core Program(JSJSCCB20200010).

摘　　要：Background Opisthorchiid flukes,particularly Opisthorchis viverrini,Opisthorchis felineus,Clonorchis sinensis,and Metorchis spp.are the most common fish-borne zoonotic human liver flukes(hLFs).Liver fluke infections are more prevalent in resource-deprived and underprivileged areas.We herein estimated the prevalence of the metacercariae(MC)of major hLFs in common large freshwater fishes(lFWF)marketed for human consumption from some selected areas of Bangladesh along with detection of their molluscan vectors and reservoirs.Methods The current status of fish-borne zoonotic hLF infections in lFWF was investigated along with their molluscan vectors and mammalian reservoir hosts in Mymensingh and Kishoreganj in Bangladesh from July 2018-June 2022 using conventional and multiple molecular techniques,such as PCR,PCR-restriction fragment length polymorphism(RFLP),sequencing,and bioinformatic analyses.The infection rate of fishes was analyzed using the Z-test and the loads of MC were compared using the chi-squared(χ^(2))test.Results The MC of C.sinensis,Opisthorchis spp.,and Metorchis spp.were detected in 11 species of common and popular lFWF.In lFWF,the estimated prevalence was 18.7%and the mean load was 137.4±149.8 MC per 100 g of fish.The prevalence was the highest(P<0.05)in spotted snakehead fishes(Channa punctata,63.6%).The highest rate of infection(P<0.05)was observed with the MC of C.sinensis(11.8%).Metacercariae were almost equally(P>0.05)distributed between the head and body of fishes.The infection rate was slightly higher in cultured(19.6%)fishes.The MC of C.sinensis,O.felineus,O.viverrini,and Metorchis orientalis in fishes were confirmed using PCR,PCR-RFLP and bioinformatics.The cercariae of opisthorchiid(Pleurolophocercus cercariae)flukes were only recovered from Bithynia spp.(3.9%,42 out of 1089).The ova of hLFs from dogs(4.3%,5 out of 116)and cats(6.0%,6 out of 100),and adult flukes(M.orientalis)from ducks(41.1%113 out of 275)were detected.Conclusions The MC of hLFs are highly prevalent in fresh water fishe

关 键 词：Human liver fluke Freshwater fish Molluscan vector Reservoir host BANGLADESH 

分 类 号：R532.2[医药卫生—内科学] R181.3[医药卫生—临床医学]