Reconstitution of double-negative T cells after cord blood transplantation and its predictive value for acute graft-versus-host disease  

作　　者：Tianzhong Pan Peng Ding Aijie Huang Baolin Tang Kaidi Song Guangyu Sun Yue Wu Shiying Yang Xingchi Chen Dongyao Wang Xiaoyu Zhu 

机构地区：[1]Department of Hematology,The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui 230001,China [2]Anhui Provincial Key Laboratory of Blood Research and Applications,Hefei,Anhui,230001,China [3]Blood and Cell Therapy Institute,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui 230001,China [4]Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui 230001,China [5]The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui 230001,China

出　　处：《Chinese Medical Journal》2024年第10期1207-1217,共11页中华医学杂志（英文版）

基　　金：National Natural Science Foundation of China(Nos.82270223,82170209,and 82100230);Anhui Provincial Key Research and Development Project(No.2022e07020015);Anhui Health Research Project(No.AHWJ2022a 011);Fundamental Research Funds for the Central Universities(Nos.WK9110000204,and WK9110000168)

摘　　要：Background:With an increasing number of patients with hematological malignancies being treated with umbilical cord blood transplantation(UCBT),the correlation between immune reconstitution(IR)after UCBT and graft-versus-host disease(GVHD)has been reported successively,but reports on double-negative T(DNT)cell reconstitution and its association with acute GVHD(aGVHD)after UCBT are lacking.Methods:A population-based observational study was conducted among 131 patients with hematological malignancies who underwent single-unit UCBT as their first transplant at the Department of Hematology,the First Affiliated Hospital of USTC,between August 2018 and June 2021.IR differences were compared between the patients with and without aGVHD.Results:The absolute number of DNT cells in the healthy Chinese population was 109(70-157)/μL,accounting for 5.82(3.98-8.19)%of lymphocytes.DNT cells showed delayed recovery and could not reach their normal levels even one year after transplantation.Importantly,the absolute number and percentage of DNT cells were significantly higher in UCBT patients without aGVHD than in those with aGVHD within one year(F=4.684,P=0.039 and F=5.583,P=0.026,respectively).In addition,the number of DNT cells in the first month after transplantation decreased significantly with the degree of aGVHD increased,and faster DNT cell reconstitution in the first month after UCBT was an independent protective factor for aGVHD(HR=0.46,95%confidence interval[CI]:0.23-0.93;P=0.031).Conclusions:Compared to the number of DNT cells in Chinese healthy people,the reconstitution of DNT cells in adults with hematological malignancies after UCBT was slow.In addition,the faster reconstitution of DNT cells in the early stage after transplantation was associated with a lower incidence of aGVHD.

关 键 词：Umbilical cord blood transplantation Graft-versus-host disease Immune reconstitution Double negative T Hematological Malignancies Treg cell Breg cell 

分 类 号：R457[医药卫生—治疗学]