通过超分辨率超快超声技术观测不同节段SD大鼠脊髓损伤后血管血流改变特征  

Application of super-resolution and ultrafast ultrasound to reveal the characteristics of vascular blood flow changes after rat spinal cord injury at different segments

作　　者：董浩如 郁钧瑾 陈星宇 许凯亮 谢嵘 Dong Haoru;Yu Junjin;Chen Xingyu;Xu Kailiang;Xie Rong(Department of Neurosurgery,Huashan Hospital,Fudan University,Shanghai 200040,China;School of Information Science and Engineering,Fudan University,Biomedical Engineering Center,Shanghai 200438,China)

机构地区：[1]复旦大学附属华山医院神经外科,上海200040 [2]复旦大学信息科学与工程学院生物医学工程中心,上海200438

出　　处：《中华医学杂志》2024年第9期690-694,共5页National Medical Journal of China

基　　金：国家自然科学基金(82071315,82271342);复旦大学2022年度医工结合项目(XM03231517)

摘　　要：目的通过超分辨率超快超声技术观测颈、胸、腰不同节段脊髓损伤后SD大鼠脊髓血管血流的变化情况。方法共9只SD大鼠使用50 g动脉瘤夹损伤脊髓来构建不同节段大鼠脊髓损伤模型,损伤前及损伤后6 h使用超分辨率超快超声技术对损伤前后的SD大鼠脊髓进行血管血流成像,得到脊髓血管密度、血流速度等定量信息。结果超声成像显示,脊髓损伤后胸段血管密度下降幅度(18.16%±1.04%)多于颈段(11.42%±1.39%)与腰段(13.88%±1.43%)(均P<0.05);胸段发生血管密度下降的脊髓长度[(4.80±0.34)mm]长于颈段[(2.80±0.57)mm]与腰段[(3.10±0.36)mm,均P<0.05];损伤后胸段血流量下降[(8.87±0.85)ml/min]高于颈段[(4.88±0.56)ml/min]与腰段[(6.19±0.71)ml/min,均P<0.05]。HE染色与尼氏染色显示胸段脊髓损伤后空腔面积占比(11.53%±0.93%)高于颈段(4.90%±1.72%)和腰段(7.64%±0.84%)(均P<0.05),且胸段尼氏体数量(18.0±5.3)也少于颈段(32.3±5.1)与腰段(37.0±5.6)脊髓损伤(均P<0.05)。结论不同节段SD大鼠脊髓损伤后血管血流改变存在不同,相同损伤在胸段脊髓对血管的破坏最严重,腰段脊髓次之,颈段脊髓损坏最小。Objective To investigate the changes of spinal vascular blood flow in SD rats after cervical,thoracic and lumbar spinal cord injury(SCI)using super-resolution ultrafast ultrasound technology.Methods A total of 9 SD rats were used to construct SCI models at different segments using a 50 g aneurysm clip.Super-resolution ultrafast ultrasound technology was used to perform vascular blood flow imaging on the spinal cord of rats before and after injury at 6 hours,obtaining quantitative information such as spinal cord vascular density and blood flow velocity.Results Ultrasound imaging showed that after SCI,the vascular density in the thoracic segment decreased(18.16%±1.04%)more than in the cervical segment(11.42%±1.39%)and lumbar segment(13.88%±1.43%,both P<0.05).The length of the spinal cord with decreased vascular density in the thoracic segment[(4.80±0.34)mm]was longer than that in the cervical segment[(2.80±0.57)mm]and lumbar segment[(3.10±0.36)mm,both P<0.05].After injury,the decrease of blood flow in the thoracic segment[(8.87±0.85)ml/min]was higher than that in the cervical segment[(4.88±0.56)ml/min]and lumbar segment[(6.19±0.71)ml/min,both P<0.05].HE staining and Nissl staining showed that the proportion of cavity area after thoracic SCI(11.53%±0.93%)was higher than that in the cervical segment(4.90%±1.72%)and lumbar segment(7.64%±0.84%,both P<0.05).The number of Nissl bodies in the thoracic segment(18.0±5.3)was also lower than that in the cervical segment(32.3±5.1)and lumbar segment(37.0±5.6)(both P<0.05).Conclusions There are different changes in vascular blood flow after SCI in different segments of rats.The same injury causes the most severe damage to blood vessels in the thoracic spinal cord,followed by the lumbar spinal cord,and the cervical spinal cord has the least damage.

关 键 词：脊髓损伤 脊髓血管血流 超分辨率超快超声 大鼠 

分 类 号：R651.2[医药卫生—外科学]