ALDH2基因多态性与老年2型糖尿病发生及冠状动脉狭窄病变的相关性分析  

Aldehyde dehydrogenase 2 gene polymorphism and its correlation with the occurrence of type 2 diabetes mellitus and coronary stenosis in the elderly:a retrospective study

作　　者：蔡国强[1] 马冬纹[1] 徐娟 徐佟 刘新梅[1] Cai Guoqiang;Ma Dongwen;Xu Juan;Xu Tong;Liu Xinmei(Cardiology Intensive Care Medicine Department,the Affiliated Hospital of Jining Medical University,Jining 272000,China)

机构地区：[1]济宁医学院附属医院心脏重症医学科,济宁272000

出　　处：《中国医师杂志》2023年第10期1530-1533,共5页Journal of Chinese Physician

基　　金：济宁市重点研发计划项目(2020JKNS007)

摘　　要：目的观察老年2型糖尿病(T2DM)患者的乙醛脱氢酶2(ALDH2)基因多态性并分析其与冠状动脉(简称冠脉)狭窄病变的相关性。方法选取2020年3月至2022年3月在济宁医学院附属医院收治的94例老年T2DM患者作为观察组,并选取与之匹配的健康体检人群50例作为对照组,观察组根据其冠脉是否存在狭窄分为狭窄组和无狭窄组。收集入组者临床资料,留取入组者血清标本,采用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR-RFLP)检测ALDH2基因多态性(rs671位点),观察老年T2DM患者ALDH2基因多态性分布,利用多因素logistic回归分析影响老年T2DM患者发生冠脉狭窄的独立因素;比较不同基因型狭窄组患者冠脉病变程度。结果观察组ALDH2基因(rs671位点)等位基因A频率高于对照组(P<0.05);狭窄组糖尿病病程≥5年,有吸烟史的占比均高于无狭窄组(均P<0.05);狭窄组血清GLU、TC、LDL-C水平高于无狭窄组,HDL-C低于无狭窄组(均P<0.05)。狭窄组ALDH2基因(rs671位点)基因型AA占比、等位基因A频率高于无狭窄组(均P<0.05)。糖尿病病程长、低水平HDL-C、ALDH2基因(rs671位点)基因型AA是影响老年T2DM患者并发冠脉狭窄的独立危险因素(均P<0.05);基因型AA的狭窄组患者三支及以上的冠脉病变占比均较基因型GG和GA高(均P<0.05)。结论ALDH2基因多态性与老年患者T2DM的发生有关;等位基因A携带者发生冠脉狭窄的风险更高,病情更重。Objective To investigate the allele distribution of aldehyde dehydrogenase 2(ALDH2)gene polymorphism in elderly patients with type 2 diabetes mellitus(T2DM)and to analyze its correlation with the presence and severity of coronary stenosis.Methods A total of 94 elderly patients with T2DM who were admitted to the Affiliated Hospital of Jining Medical University from March 2020 to March 2022 were selected as the observation group,and 50 age-gender-matched healthy subjects were selected as the control group.The observation group was further divided into stenosis group and non-stenosis group based on the presence of coronary stenosis.Clinical data were collected from all participants,and blood samples were taken for analysis.The ALDH2 gene polymorphism(rs671 locus)was detected using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)technology.The distribution of ALDH2 genotypes was observed in elderly patients with T2DM,and multivariate logistic regression analysis was used to identify independent factors associated with the development of coronary stenosis in elderly patients with T2DM.The degree of coronary stenosis was compared between patients with different genotypes.Results The allele A frequency of ALDH2 gene(rs671 locus)was significantly higher in the observation group than in the control group(P<0.05).The proportion of patients with diabetes duration≥5 years and smoking history in the stenosis group was significantly higher than that in the non-stenosis group(all P<0.05).Serum levels of glucose(GLU),total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C)were significantly higher in the stenosis group than in the non-stenosis group,while high-density lipoprotein cholesterol(HDL-C)level was significantly lower in the stenosis group than in the non-stenosis group(all P<0.05).The proportion of ALDH2 genotypes AA and allele A frequency were significantly higher in the stenosis group than in the non-stenosis group(all P<0.05).Long duration of diabetes,low HDL-C level,and ALD

关 键 词：糖尿病 2型 冠状动脉狭窄 乙醛脱氢酶2 多态现象 遗传 

分 类 号：R587.2[医药卫生—内分泌] R541.4[医药卫生—内科学]