Single-cell RNA sequencing reveals the transcriptomic landscape of kidneys in patients with ischemic acute kidney injury  

作　　者：Rong Tang Peng Jin Chanjuan Shen Wei Lin Leilin Yu Xueling Hu Ting Meng Linlin Zhang Ling Peng Xiangcheng Xiao Peter Eggenhuizen Joshua DOoi Xueqin Wu Xiang Ding Yong Zhong 

机构地区：[1]Department of Nephrology,Xiangya Hospital,Central South University,Changsha,Hunan 410008,China [2]Key Laboratory of Biological Nanotechnology of National Health Commission,Xiangya Hospital,Central South University,Changsha,Hunan 410008,China [3]Department of Organ Transplantation,Xiangya Hospital,Central South University,Changsha,Hunan 410008,China [4]Department of Hematology,The Affiliated Zhuzhou Hospital Xiangya Medical College,Central South University,Zhuzhou,Hunan 412007,China [5]Department of Pathology,Xiangya Hospital,Central South University,Changsha,Hunan 410008,China [6]Department of Nephrology,Jiujiang Hospital of Traditional Chinese Medicine,Jiujiang,Jiangxi 332099,China [7]Department of Medicine,Centre for Inflammatory Diseases,Monash Medical Centre,Monash University,Clayton,VIC 3168,Australia [8]Department of Nephrology,The Third Xiangya Hospital,Central South University,Changsha,Hunan 410013,China [9]National Clinical Research Center for Geriatric Disorders,Xiangya Hospital,Central South University,Changsha,Hunan 410008,China

出　　处：《Chinese Medical Journal》2023年第10期1177-1187,共11页中华医学杂志（英文版）

基　　金：National Key Research and Development Program of China(No.2020YFC2005000);Key Research and Development Program of Hunan province(No.2020WK2008);science and technology innovation Program of Hunan Province(No.2020RC5002);Natural Science Foundation of Hunan Province(Nos.2022JJ30070,2021JJ31130 and 2021JJ31057);Project of Health Commission of Hunan Province(Nos.A202303050036 and 202104101009);"Yiluqihang Shenmingyuanyang"medical development and Scientific Research Fund project on Kidney Diseases(No.SMYY20220301001)

摘　　要：Background:Ischemic acute kidney injury(AKI)is a common syndrome associated with considerable mortality and healthcare costs.Up to now,the underlying pathogenesis of ischemic AKI remains incompletely understood,and specific strategies for early diagnosis and treatment of ischemic AKI are still lacking.Here,this study aimed to define the transcriptomic landscape of AKI patients through single-cell RNA sequencing(scRNA-seq)analysis in kidneys.Methods:In this study,scRNA-seq technology was applied to kidneys from two ischemic AKI patients,and three human public scRNA-seq datasets were collected as controls.Differentially expressed genes(DEGs)and cell clusters of kidneys were determined.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis,as well as the ligand-receptor interaction between cells,were performed.We also validated several DEGs expression in kidneys from human ischemic AKI and ischemia/reperfusion(I/R)injury induced AKI mice through immunohistochemistry staining.Results:15 distinct cell clusters were determined in kidney from subjects of ischemic AKI and control.The injured proximal tubules(PT)displayed a proapoptotic and proinflammatory phenotype.PT cells of ischemic AKI had up-regulation of novel pro-apoptotic genes including USP47,RASSF4,EBAG9,IER3,SASH1,SEPTIN7,and NUB1,which have not been reported in ischemic AKI previously.Several hub genes were validated in kidneys from human AKI and renal I/R injury mice,respectively.Furthermore,PT highly expressed DEGs enriched in endoplasmic reticulum stress,autophagy,and retinoic acid-inducible gene I(RIG-I)signaling.DEGs overexpressed in other tubular cells were primarily enriched in nucleotide-binding and oligomerization domain(NOD)-like receptor signaling,estrogen signaling,interleukin(IL)-12 signaling,and IL-17 signaling.Overexpressed genes in kidney-resident immune cells including macrophages,natural killer T(NKT)cells,monocytes,and dendritic cells were associated with leukocyte activation,chemotaxis,cell adhesion,a

关 键 词：Ischemic acute kidney injury Single-cell RNA sequencing Proximal tubule cells Apoptosis Inflammation Cell-cell crosstalk 

分 类 号：R692[医药卫生—泌尿科学]