含中药三七的壳聚糖/明胶水凝胶复合止血材料的制备和性能评价  

Preparation and performance evaluation on chitosan/gelatin hydrogel hemostatic material containing

作　　者：支海宁 邵荣江[1,2] 郭维 王传林[3,4,5] 李明 Zhi Haining;Shao Rongjiang;Guo Wei;Wang Chuanlin;Li Ming(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300073,China;National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300073,China;Trauma Medicine Center,Peking University People’s Hospital,Beijing 100044,China;National Center for Trauma Medicine,Beijing 100044,China;Key Laboratory of Trauma and Neural Regeneration,Ministry of Education,Beijing 100044,China)

机构地区：[1]天津中医药大学第一附属医院,天津300073 [2]国家中医针灸临床医学研究中心,天津300073 [3]北京大学人民医院创伤救治中心,北京100044 [4]国家创伤医学中心,北京100044 [5]创伤救治与神经再生教育部重点实验室,北京100044

出　　处：《国际生物医学工程杂志》2023年第1期48-54,共7页International Journal of Biomedical Engineering

摘　　要：目的制备负载中药三七的壳聚糖/明胶水凝胶复合止血材料(PN/CMC/GMs),并对其性能进行评价。方法利用冷冻干燥法制备PN/CMC/GMs,利用扫描电镜观察其形态,流变仪观察其流变学性能,溶胀测试其吸水膨胀率,细胞毒性实验检测其生物相容性,并利用SD大鼠肝脏出血模型检测其快速止血效果。结果制备了PN/CMC/GMs,呈网格状结构,具有一定的孔隙率。随着三七粉含量的增加,PN/CMC/GMs的模量也相应的增加,机械强度增加。PN/CMC/GMs具有较好的吸水膨胀功能,可形成压迫止血和浓缩血液实现快速止血,且具有良好的生物相容性。止血实验表明,PN/CMC/GMs对大鼠肝脏损伤的止血时间和止血效果均优于空白对照组。结论PN/CMC/GMs具有良好的止血效果和生物相容性,具有进一步研究的价值和临床应用前景。Objective To prepare chitosan/gelatin hydrogel composite hemostatic materials loaded with Panax notoginseng(PN/CMC/GMs)and evaluate their performance.Methods PN/CMC/GMs hydrogel composite hemostatic material were prepared by the freeze-drying method,and their morphology was observed by scanning electron microscopy.Their rheological properties were observed by a rheometer.Their water absorption rate was tested by dissolution.Their biocompatibility was detected by a cytotoxicity assay.Their rapid hemostatic effect was tested using a SD rat liver hemorrhage model.Results PN/CMC/GMs composite hemostatic materials were prepared in a lattice-like structure with certain porosity.With the increase in Panax notoginseng powder content,the modulus of PN/CMC/GMs increased accordingly,and the mechanical strength increased.PN/CMC/GMs have better water absorption and expansion functions,which can form compression hemostasis and concentrated blood to achieve rapid hemostasis,and have good biocompatibility.Hemostasis experiments showed that the hemostatic time and hemostatic effect of PN,CMC/GMs hemostatic materials on liver injury in rats were better than those of the blank control group.Conclusions PN/CMC/GMs have good hemostatic effect and biocompatibility and have the potential for further research and clinical application.

关 键 词：壳聚糖 明胶 三七 水凝胶 止血材料 

分 类 号：R318.08[医药卫生—生物医学工程]