中亚苦蒿醇提物大孔树脂30%乙醇洗脱部位免疫功能研究  

Immune enhancement of 30% ethanol elution fraction of Artemisia absinthium ethanol extract with macroporous resin

作　　者：马梓航 李金耀[1] 杨兴龙 蒋静雯 陈秋艳 夏丽洁[1] Ma Zihang;Li Jinyao;Yang Xinglong;Jiang Jingwen;Chen Qiuyan;Xia Lijie(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《国际生物医学工程杂志》2023年第1期10-17,共8页International Journal of Biomedical Engineering

基　　金：国家自然科学基金(31860258);国家级大学生创新训练计划项目(202010755041)

摘　　要：目的探讨中亚苦蒿醇提物大孔树脂30%乙醇洗脱部位(AAEM-30%)对小鼠树突状细胞(DC)和免疫功能的作用。方法中亚苦蒿醇提物经AB-8型大孔树脂分离得到AAEM-30%,对其多糖、黄酮和萜类含量进行测定。体外通过流式细胞术检测AAEM-30%对DC表面分子分化簇(CD)40、CD80和CD86的表达,酶联免疫吸附测定(ELISA)法检测DC细胞因子白细胞介素(IL)-6和肿瘤坏死因子-α(TNF-α)的表达;体内通过不同给药剂量(50、100 mg/kg)和不同给药方式(皮下注射、腹腔注射、灌胃)检测AAEM-30%对ICR小鼠免疫功能的影响。结果AAEM-30%中多糖、黄酮、萜类的质量分数分别为24.30%、22.50%、28.19%。体外能够增强小鼠DC表面分子CD40、CD86以及DC细胞因子IL-6和TNF-α的表达(均P<0.001)。与对照组比较,3种给药方式的小鼠体质量比较差异均无统计学意义(均P>0.05);50、100 mg/kg AAEM-30%腹腔注射组的胸腺指数和50 mg/kg AAEM-30%灌胃组的脾脏指数均增加(均P<0.05)。100 mg/kg AAEM-30%腹腔注射组的CD19^(+)细胞数量增加(P<0.01),50 mg/kg AAEM-30%灌胃组的CD19^(+)细胞数量增加(P<0.05)。100 mg/kg AAEM-30%灌胃组的CD11b^(+)和CD11c^(+)数量增加(P<0.05)。灌胃和腹腔注射2种给药方式均能增加CD4^(+)和CD8^(+)T淋巴细胞的数量(均P<0.05)。结论AAEM-30%能够促进小鼠DC的成熟,增强小鼠免疫功能。Objective To investigate the effects of 30%ethanol elution fraction of Artemisia absinthium extract with macroporous resin(AAEM-30%)on the dendritic cell(DC)and immunity of mice.Methods AAEM-30%was obtained from the alcoholic extracts of A.absinthium by AB-8 macroporous resin,and its polysaccharide,flavonoid,and terpenoid contents were determined.The expressions of AAEM-30%on DC surface molecular cluster of differentiation(CD)40,CD80 and CD86 were detected in vitro by flow cytometry,and the expressions of DC cytokines IL-6 and tumor necrosis factor-α(TNF-α)were detected by enzyme linked immunosorbent assay(ELISA).The effect of AAEM-30%on the immune function of ICR mice was measured in vivo with different doses(50 and 100 mg/kg)and different administration methods(subcutaneous injection,intraperitoneal injection,and gavage).Results The contents of polysaccharides,flavonoids,and terpenoids in AAEM-30%were 24.30%,22.50%and 28.19%,respectively.AAEM-30%significantly enhanced the expression of CD40,and CD86 and the secretion of IL-6 and TNF-α(all P<0.001).Compared with the control group,no statistically significant differences were found in the body mass of mice compared with the three administration methods(all P>0.05).The thymus index in the 50 and 100 mg/kg AAEM-30%intraperitoneal injection groups and the spleen index in the 50 mg/kg AAEM-30%gavage group were increased(all P<0.05).CD19^(+)cells increased in the 100 mg/kg AAEM-30%intraperitoneal injection group(P<0.01)and in the 50 mg/kg AAEM-30%gavage group(P<0.05).The CD11b^(+)and CD11c^(+)counts increased in the 100 mg/kg AAEM-30%gavage group(P<0.05).The number of CD4^(+)and CD8^(+)T lymphocytes was increased by both gavage and intraperitoneal administration(all P<0.05).Conclusions AAEM-30%can promote the maturation of DC and enhanced the immunity of mice without obvious side effects.

关 键 词：中亚苦蒿醇提物 树突状细胞 免疫功能 

分 类 号：R285[医药卫生—中药学]