人血浆毒鼠强气相色谱-氮磷检测器检测及其临床应用  

The Determination of Tetramine in Human Plasma by GC/NPD and Clinical Application

作　　者：杜云波[1] 王汉斌[2] 王浩春[2] 赵英萍[1] 

机构地区：[1]广东省江门市中心医院ICU,529070 [2]北京军事医学科学院附属医院,全军中毒救治专科中心

出　　处：《岭南急诊医学杂志》2007年第4期292-293,共2页Lingnan Journal of Emergency Medicine

摘　　要：目的:利用气相色谱-氮磷检测器(GC/NPD)建立一种快速检测人血浆中毒鼠强(TET)的方法。方法:血浆中TET用乙酸乙酯作液液提取,毛细管气相色谱柱,GC/NPD法测定血浆中毒鼠强的浓度,以甲基1605为内标,内标法定量。结果:方法学显示毒鼠强与内标分离良好,不受样品中内源性物质干扰,在50～700ng/mL范围内具有良好的线性关系(r>0.99),最低检出浓度为5ng/mL,最低检测限为10ng/mL。血浆样本回收率的在70%以上。日内、日间精密度分别为RSD3.5%～12.8%和6.5%～12.0%。结论:该本方法灵敏、稳定,能满足毒鼠强药物代谢动力学研究和临床检测的需要。Objective: To build a method by gas chromatography/nitrogen-phosphorous detector (GC/NPD) quantitative technique for the determination of tetramine(TET) in human plasma. Methods: Ethyl acetate was used for extraction. A HP-5 phase capillary column was used. The detector was GC/NPD. The internal standard was Methyl 1605. Results: The chromatograms results showed that TET and inter standard 1605 were well separated from each other, and both were not disturbed by endogenesis substances; The result showed that the linearity of standard curves of sodium chloride solution were excellent(r>0.99); the average recovery of TET from sodium chloride solution was more than 70%; the inter-day and intra-day RSD were lower than 15%; the lowest qualitative determination concentration was 5 ng/mL, the lowest quantitative determination concentration was 10 ng/mL. Conclusion: The extraction and analysis method of TET established provides a suitable analytical method to toxicokinetics and clinic study of tetramine poisoning.

关 键 词：毒鼠强 气相色谱氮磷检测器法 血浆 

分 类 号：R595.4[医药卫生—内科学]