转染CⅡTA基因上调小鼠树突状细胞表面MHC分子和共刺激分子的表达  

Enhance the expression of MHC class Ⅰ/Ⅱ molecules and CD80/CD86 molecules on dendritic cells by transfection of CIITA gene

作　　者：杨滨[1] 陈静[1] 郭慈仁[1] 欧启水[1] 

机构地区：[1]福建医科大学附属第一医院检验科福建医科大学基因诊断研究室福建医科大学医学检验系,福建福州350005

出　　处：《中国实验诊断学》2007年第11期1435-1437,共3页Chinese Journal of Laboratory Diagnosis

基　　金：福建省自然科学基金资助(C0310016);福建省创新医学课题基金资助(2001-CX-04)

摘　　要：目的探讨MHCⅡ类分子反式激活因子(MHC classⅡtransactivator,CⅡTA)基因对树突状细胞(Dendriticcell,DC)表面MHC-Ⅰ/-Ⅱ类分子及共刺激分子CD80、CD86的调节作用,为将树突状细胞应用于生物治疗奠定基础。方法从小鼠骨髓中大量扩增DC,用脂质体转染法将小鼠CⅡTA(mCⅡTA)基因转入DC中,用流式细胞术观察转染前、后DC表面MHCⅠ-/-Ⅱ类分子及CD80、CD86的表达。结果转染mCⅡTA基因使DC上MHC-Ⅰ/-Ⅱ分子的表达率由74.2%/66.7%明显上调为93.6%/91.4%;CD80/CD86的表达率由52.3%/60.5%明显上调为89.7%/91.5%(P<0.05)。结论转入mCⅡTA可使DC表面MHC-Ⅰ/-Ⅱ分子及CD80/CD86的表达率明显上调。本研究为将DC应用于肿瘤的生物治疗奠定了基础。Objective To explore the effect of MHC class Ⅱ transactivator(CⅡTA) gene on the expression of MHC class Ⅰ/Ⅱ molecules,CD80 and CD86 molecules on dendritic cells(DCs).Methods We transfected the mouse CⅡTA gene(mCⅡTA) by lipofectamine into DCs drived from mouse bone marrow.The expression of MHC class Ⅰ/Ⅱ,CD80,and CD86 molecules was detected by flow cytometry.Results After transfection of mCⅡTA,the expression rate of MHC class Ⅰ/Ⅱ molecules of DCs rose from 74.2%/66.7% to 93.6%/91.4%,and that of CD80/CD86 rose from 52.3%/60.5% to 89.7%/91.5%.Conclusion The expression of MHC Ⅰ/Ⅱ,CD80 and CD86 on DCs was up-regulated markedly by transfection of mCⅡTA.Our results provided a new method of using DC in biotherapy of tumors and autoimmune disease.

关 键 词：CⅡTA 树突状细胞 MHC分子 共刺激分子 小鼠 

分 类 号：Q78[生物学—分子生物学]