芬特明片剂含量以及溶出度样品含量的反相HPLC测定  

Determination and dissolution of psychic drugs phentermine tablet by RT-HPLC

作　　者：彭波[1] 郝杰[2] 刘辽[2] 杨云霞[2] 

机构地区：[1]中山大学生命科学院动物生理教研室,广州510275 [2]四川大学华西基础与法医学院药理教研室,四川成都610041

出　　处：《四川生理科学杂志》2007年第3期109-111,共3页Sichuan Journal of Physiological Sciences

摘　　要：目的:用反相HPLC测定方法检测芬特明片剂的含量和溶出度。方法:Capcell-pakC18色谱柱,甲醇为流动相,检测波长为254nm,流速1.0ml.min-1,以咖啡因作为内标计算含量。Capcell-pakC18色谱柱,甲醇/辛烷磺酸钠离子溶液作为流动相,检测波长为208nm,流速1.0ml.min-1,外标法计算溶出度。结果:含量试验线性范围为:18.75～300mg.100ml-1,r=0.9999,回收率为100.06%,RSD为0.22%。溶出度线性范围为:1～16mg.100ml-1,r=0.9999。结论:该条件下反相HPLC测定芬特明片剂的含量、溶出度药物含量测定方法快速简便,结果满意。Objective:To establish a method for determination and dissolution of Phentermine tablets by RT-HPLC.Methods:Using RT-HPLC with Capcell-pak C18 column,methanol as the mobile phase.Detection wavelength was 254nm.Results:Calibration curves were linear in the range of 18.75～300 mg·100 ml-1 for determination and 1～16 mg·100 ml-1 for dissolution test,(r=0.9999).Conclusion:The method is simple,rapid and the results is accurate,reliable.

关 键 词：芬特明 RP-HPLC 含量测定 溶出度 

分 类 号：R927.2[医药卫生—药学]