HPLC法测定复方莫西沙星滴鼻液中2组分的含量  

Determination of 2 Kinds of Components in Compound Moxifloxacin Nasal Drops by HPLC

作　　者：黄道秋[1] 祁俊生[2] 赵雪娥[3] 张斌[1] 李柏群[1] 丁铃[1] 

机构地区：[1]重庆三峡中心医院,重庆市404000 [2]重庆三峡学院化学与环境工程系,重庆市404000 [3]重庆万州区药品检验所,重庆市404000

出　　处：《中国药房》2007年第13期1011-1012,共2页China Pharmacy

摘　　要：目的:建立以高效液相色谱法同时测定复方莫西沙星滴鼻液中盐酸莫西沙星及盐酸麻黄碱含量的方法。方法:色谱柱为VP-ODS,流动相为磷酸盐缓冲液(含0·02mol·L-1磷酸二氢钠的0·3%三乙胺水溶液,用磷酸调pH至3·0)-甲醇(60∶40),流速为1·0mL·min-1,检测波长为214nm,进样量为20μL,柱温为30℃。结果:盐酸莫西沙星与盐酸麻黄碱的线性范围分别为0·1～0·8(r=0·9997,n=5)、0·125～1·000μg(r=0·9999,n=5);平均回收率分别为98·94%(RSD=0·89%)、99·67%(RSD=1·26%)。结论:本方法简便、快速、准确、重现性好,可用于该制剂的质量控制。OBJECTIVE:To establish a HPLC method for a simultaneous determination of moxifloxacin hydrochloride and ephedrine hydrochloride in compound moxifloxacin nasal drops.METHODS:The separation was performed on VP-ODS chromatographic column with column temperature at 30℃. The mobile phase consisted of phosphate buffer solution (which contained 0.02mol·L-1 sodium dihydrogen phosphate and 0.3% triethylamine, adjust pH to 3.0 by phosphoric acid) - methanol(60∶40) with flow rate at 1.0mL·min-1, detection wavelength a...

关 键 词：高效液相色谱法 盐酸莫西沙星 盐酸麻黄碱 滴鼻液 含量测定 

分 类 号：R927[医药卫生—药学]