高效液相色谱法测定血浆中尼扎替丁浓度  

Determination of nizatidine by HPLC in human plasma

作　　者：江文明[1] 陈钧[1] 谢月玲[1] 高小玲[1] 蒋新国[1] 

机构地区：[1]复旦大学药学院药剂学教研室,上海200032

出　　处：《中国医院药学杂志》2005年第1期43-45,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的:建立测定人血浆中尼扎替丁的高效液相色谱方法。方法:采用DiamonsilC18色谱柱(200mm×4.6mm,5μm),流 动相为0.1mol·L-1醋酸铵缓冲液 甲醇(60∶40),检测波长为320nm。血浆样品加盐析溶液碱化后以氯仿提取,雷尼替丁为内标。 结果:尼扎替丁血药浓度线性范围为20～6000μg·L-1(r=0.9999,n=6),最低检测浓度为10μg·L-1(S/N=3),方法回收率在 96.84%～101.39%(n=5),日内和日间RSD均小于4%。结论:本法简便,快速,重现性好,适于尼扎替丁的药动学研究。OBJECTIVE To establish a HPLC method for the determination of nizatidine in human plasma.METHODS Nizatidine was extracted from human plasma using chloroform after basified with salting-out agent,and then separated on a Diamonsil C 18 column ( 200 mm × 4.6 mm, 5 μm).The mobile phase consisted of water( 0.1 mol · L -1 ammonium acetate)-methanol(60∶40 ). Ranitidine was used as the internal standard and the wavelength of detection was set at 320 nm.RESULTS The linear range was 20- 6 000 μg·L ...

关 键 词：尼扎替丁 高效液相色谱法 测定 

分 类 号：R96[医药卫生—药理学]