高效液相色谱法测定血浆中雷贝拉唑的浓度  

HPLC determination of raberazole in human plasma

作　　者：扈本荃[1] 周瑾[1] 程建峰[1] 贺建荣[1] 廉江平[2] 张鹏[2] 

机构地区：[1]西安第四军医大学唐都医院,陕西西安710038 [2]陕西省人民医院,陕西西安710068

出　　处：《中国医院药学杂志》2005年第1期45-47,共3页Chinese Journal of Hospital Pharmacy

摘　　要：　目的:建立高效液相色谱法测定血浆中雷贝拉唑的浓度。方法:血浆样品经处理后,选用Shim-packVP-ODS柱 (150mm×4.6mm,5μm),0.05mol·L-1磷酸二氢钾缓冲溶液(0.1mol·L-1氢氧化钠溶液调pH7.0) 乙腈(69∶31)为流动相,检 测波长为284nm。结果:雷贝拉唑血药浓度的线性范围为5.46～2184.61μg·L-1(r=0.9999),提取回收率78.25%～89.60%,方 法回收率94.56%～97.69%,日内、日间RSD分别为3.52%和4.60%。结论:该方法准确、灵敏,适于雷贝拉唑药动学研究。OBJECTIVE An RP-HPLC method was developed for detemination of raberazole in the plasma of human.METHODS The raberazole was extracted from plasma and the applied column was Shim-pack VP -ODS ( 150 mm× 4.6 mm, 5 μm).The mobile phase consisted of 0.1 mol·L -1 dihydrogen phosphate buffer solution (pH 7.0 adjusted with sodium hydroxide solution)- acetonitrile(69∶ 31).Raberazole was detected by UV absorption at 284 nm.RESULTS Standard curve was liner in the range of 5.46- 2 184.61 μg· L ...

关 键 词：高效液相色谱法 雷贝拉唑 血药浓度 

分 类 号：R96[医药卫生—药理学]