Fe_3O_4/葡聚糖/抗体磁性纳米生物探针的制备和层析检测  

Preparation of Nano-scaled Magnetic Biological Probes of Fe_3O_4/Dextran/Antibody and Chromatographic Assay

作　　者：洪霞[1] 郭薇[1] 李军[1] 刘艳梅[1] 杨文胜[1] 白玉白[1] 李铁津 袁航[2] 马岚[2] 

机构地区：[1]吉林大学化学学院,长春130023 [2]云南大学单克隆抗体工程技术研究中心,昆明650091

出　　处：《高等学校化学学报》2004年第3期445-447,394,共4页Chemical Journal of Chinese Universities

基　　金：国家自然基金项目 (批准号 :2 0 0 73 0 16;60 2 710 2 0 );国家"973"计划项目 (批准号 :G19990 64 5 0 4);"863"计划项目 (批准号 :2 0 0 2 AA3 0 2 2 0 3 )资助

摘　　要：Dextran coated magnetic nanocomposites were prepared. A periodate oxidation method was used to further activate the magnetic dextran, forming magnetic polyaldehyde dextran, which could be conjugated to biomolecules, antibodes. The magnetic labeled nano scaled bioconjugates could be well blocked with BSA and exhibit high activity by ELISA and immunochromatographic strip assay.Dextran coated magnetic nanocomposites were prepared. A periodate oxidation method was used to further activate the magnetic dextran, forming magnetic polyaldehyde dextran, which could be conjugated to biomolecules, antibodes. The magnetic labeled nano scaled bioconjugates could be well blocked with BSA and exhibit high activity by ELISA and immunochromatographic strip assay.

关 键 词：磁性生物探针 抗原-抗体识别 BSA封闭 层析检测 

分 类 号：O658[理学—分析化学]