多壁碳纳米管(MWNTs)对梨形四膜虫的生长促进作用  

Observation of growth stimulation of Tetrahymena pyriformis exposed to MWNTs

作　　者：诸颖[1] 冉铁成[1] 李宇国[1] 李文新[1] 

机构地区：[1]中国科学院上海应用物理研究所纳米生物医药研究室

出　　处：《核技术》2007年第8期689-693,共5页Nuclear Techniques

基　　金：国家自然科学基金(10475109)资助;上海市纳米专项(No.0552nm033和0652nm016);MOST973(No.2006CB705600)项目

摘　　要：本工作用显微技术观察到了四膜虫对多壁碳纳米管(MWNTs)的大量摄取。考察了四膜虫存活率与MWNTs的剂量效应,发现MWNTs对细胞的生长有浓度依赖的促进作用。四膜虫的生长刺激通过琥珀酸脱氢酶(SDH),蛋白质和核酸含量的测定被进一步证实。TGA分析表明纳米管非共价结合蛋白,因此推测,培养液中的MWNTs载带了大量蛋白胨进入四膜虫细胞内是导致四膜虫迅速增殖的原因。The uptake of multi-walled carbon nanotubes(MWNTs) by Tetrahymena pyriformis was observed with optical microscope. The viability of Tetrahymena pyriformis and its dose-effect relationship at various concentrations of the MWNTs were measured. The result revealed for the first time that MWNTs exhibited a dose-dependent growth stimulation to the cells. The rapid proliferation of cells was further confirmed by levels of the succinate dehydrogenase (SDH),protein and nucleic acid content per 104 cells of control ...

关 键 词：梨形四膜虫 多壁碳纳米管 生长促进 存活率 蛋白胨 

分 类 号：Q95-33[生物学—动物学]