间充质干细胞联合输注促进造血干细胞移植后的造血重建  

The Role of mesenchymal stem cells in hematopoietic stem cells transplantation

作　　者：施小凤[1] 傅晋翔[1] 李军[1] 陈萍[1] 袁育青[1] 黄海雯[1] 孙谕[1] 

机构地区：[1]苏州大学附属第二医院血液科,215004

出　　处：《中华器官移植杂志》2005年第7期411-414,共4页Chinese Journal of Organ Transplantation

基　　金：江苏省135重点医学人才基金资助(RC2002021);苏州大学医学发展基金资助(EE123002)

摘　　要：目的探讨间充质干细胞(MSCs)与造血干细胞(HSCs)共同移植对造血重建的影响。方法将扩增的Balb/c小鼠骨髓MSCs与骨髓共同经尾静脉输入经致死剂量照射的同系小鼠体内(联合输注组),并设单输骨髓细胞的HSCs组、单输间充质干细胞的MSCs组以及输培养基的对照组,每隔5d计数白细胞,计算动物死亡率。将乙酰乙酸碳氧荧光素标记的人间充质干细胞输入SCID小鼠尾静脉,24h后取肺、心、肝、脾、肾和小肠组织做冰冻切片;取骨髓、肝脏及脾脏,制成单细胞悬液,涂片,在荧光显微镜下观察计数;用逆转录聚合酶链反应测定鼠骨髓中人特异性β2-微球蛋白。结果细胞输注后,联合移植组的白细胞恢复快,12d左右恢复正常,死亡率(3/11)低于HSCs组(5/10)、MSCs组(4/4)、输培养基的对照组(11/11)。人MSCs输入SCID小鼠后24h,其在体内的分布依次为肺、肾、脾、肝,小肠及心脏组织中几乎无阳性细胞,骨髓中有极少MSCs。结论MSCs与造血干细胞共输能促进造血恢复。Objective To investigate the effect of mesenchymal stem cells (MSCs) on hematopoiesis during hematopoietic stem cells transplantation (HSCT) and the mechanism. Methods Cultured MSCs plus HSCs were injected intravenously into Balb/c mice irradiated lethally. Mortality rate and leukocytes were counted every 5 days. MSCs were tagged with CFDA SE and injected intravenously into SCID mice. Twenty-four h after transfusion the animals were sacrificed. The smears from liver, spleen, bone marrow and the frozen secti...

关 键 词：间充质干细胞 造血干细胞 移植 

分 类 号：R459.7[医药卫生—急诊医学]