酚酞敏化共振光散射法测定DNA  被引量:3

Determination of DNA by Resonance Light Scattering Method Using Phenothalein as a Sensitizer

在线阅读下载全文

作  者:龙云飞[1] 蒋文军[1] 陈小明[2] 苏界殊[1] 李颖[1] 

机构地区:[1]湖南科技大学化学化工学院,湘潭411201 [2]湘潭大学化学学院

出  处:《应用化学》2004年第11期1187-1189,共3页Chinese Journal of Applied Chemistry

基  金:湖南省自然科学基金(00JJY2084);湖南省教育厅基金(02C462)资助项目

摘  要:Phenothalein deoxyribonucleic acid(DNA) cetyltrimethyl ammonium bromide(CTMAB) system has been studied by resonance light scattering(RLS). In Tris HCl buffer solution(pH=9 30), phenothalein and DNA react with CTMAB to form large particles of ion association complex, which results in significant enhancement of RLS intensity. The spectral characteristics of RLS, the effective factors and optimum conditions of the reaction have been investigated. Under optimum conditions, the enhanced RLS intensity was proportional to the concentration of DNA in the range of 0 04 to 0 8 mg/L for fish sperm DNA( fsDNA ) and 0 03 to 2 5 mg/L for calf thymus DNA(ctDNA). The detection limits were 12 0 μg/L and 17 0 μg/L for fsDNA and ctDNA, respectively.Phenothalein deoxyribonucleic acid(DNA) cetyltrimethyl ammonium bromide(CTMAB) system has been studied by resonance light scattering(RLS). In Tris HCl buffer solution(pH=9 30), phenothalein and DNA react with CTMAB to form large particles of ion association complex, which results in significant enhancement of RLS intensity. The spectral characteristics of RLS, the effective factors and optimum conditions of the reaction have been investigated. Under optimum conditions, the enhanced RLS intensity was proport...

关 键 词:DNA 共振光散射 溴化十六烷基三甲基铵(CTMAB) 酚酞 定量检测 

分 类 号:Q52[生物学—生物化学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象