茶属植物及茶多酚类成分对抗原诱导RBL-2H3细胞过敏反应的抑制作用  

Inhibitory Effects of Tea Plants and Catechins on Antigen-induced Allergic Reaction in RBL-2H3 Cells

作　　者：王启隆[1] 袁丹[1] 

机构地区：[1]沈阳药科大学,辽宁沈阳110016

出　　处：《中国现代中药》2008年第1期8-11,22,共5页Modern Chinese Medicine

摘　　要：目的:研究茶属植物及茶多酚类和嘌呤碱类成分对抗原刺激RBL-2H3细胞脱颗粒及肿瘤坏死因子α(TNF-α)和白细胞介素4(IL-4)释放的抑制作用。方法:测定β-hexosaminidase的释放以评价脱颗粒反应的指标,酶联免疫吸附法测定TNF-α和IL-4的释放,评价茶中主要成分的抗过敏活性。结果:茶属植物均有抑制RBL-2H3细胞脱颗粒作用,其中花类表现出较叶类更强的抗过敏活性;EGCG对抗原刺激的RBL-2H3细胞即刻相(IC50:234μmol·L-1)和延缓相反应(IL4,IC50:158μmol·L-1),及钙离子载体A23187诱导的脱颗粒反应均有抑制作用(IC50:126μmol·L-1)。结论:茶属植物和EGCG具有明显的抗过敏作用。Objective:To investigate the effects of extract of tea plants,catechins and purine bases on degranulation and releases of TNF-α and IL-4 in rat basophilic leukemia(RBL-2H3)cells induced by antigen.Methods:To determine the release of β-hexosaminidase,as a marker of degranulation,and to examine releases of TNF-α and IL-4 by ELISA.Results:Tea plants inhibited the degranulation in RBL-2H3 cell,and extract of tea flower showed more potent activity than that of leaf.EGCG executed inhibitory activity on degranulat...

关 键 词：茶属植物 EGCG RBL-2H3细胞 脱颗粒 肿瘤因子α 白细胞介素4 

分 类 号：R284[医药卫生—中药学]