槐定碱对大鼠海马CA3区神经元超微结构的影响  

The Effect of Sorphoridine on Neuron Ultrastructure in CA3 of Hippocampus in Rats

作　　者：李玉香[1] 王洁[2] 王淑静[2] 张焱[2] 朱万平[3] 陈晓霞[1] 白洁[1] 

机构地区：[1]宁夏医学院生理教研室,宁夏银川750004 [2]宁夏医学院中心实验室,宁夏银川750004 [3]宁夏医学院组织学及胚胎学教研室,宁夏银川750004

出　　处：《中药材》2008年第1期94-97,共4页Journal of Chinese Medicinal Materials

基　　金：宁夏医学院科研项目([2007]6);宁夏自然科学基金(NZ0784)

摘　　要：目的:观察槐定碱对大鼠脑电、行为的改变及其对海马CA3区神经元超微结构的影响。方法:采用电生理学方法观察槐定碱对清醒大鼠自由活动时行为的影响,并记录脑电图;采用电镜观察给予槐定碱0.5、1.5、8 h后,大鼠海马CA3区神经元线粒体和细胞核超微结构的改变。结果:对照组大鼠未见痫样脑电波型及痫性行为,海马CA3区神经元细胞超微结构正常。槐定碱组大鼠腹腔注射槐定碱(55 mg/kg)后,海马区出现痫样放电并伴有惊厥行为发作,在电镜下可见各时间点海马CA3区神经元不同程度线粒体嵴肿胀及轴突末梢变性等改变,以1.5 h组表现最为明显。结论:槐定碱可诱发大鼠出现急性癫痫发作,并出现线粒体超微结构的损伤,其损伤早于细胞核的改变,提示线粒体的损伤可能是槐定碱引起神经元损伤的关键环节。Objective:To observe the effect of sorphoridine(SR)on electroencephalograph(EEG)and ethological changes as well as neurons ultrastructure alterations in CA3 of hippocampus in rats.Methods: Tiny electrodes were embedded into hippocampus of male SD rats.The EEG and behavior changes were observed simultaneously and ultrastructure of morphological deformation of neurons in CA3 of hippocampus were observed with electron microscope at 0.5,1.5,8 hours after SR were injected intraperitoneally on freely moving rats....

关 键 词：槐定碱 癫痫 行为学 海马 超微结构 

分 类 号：R285[医药卫生—中药学]