四种抗癌中药提取物对大鼠肝CYP3A酶活性及mRNA表达的影响  

Effects on the Activities and mRNA Expression of CYP3A in Rat's Liver by Four Kinds of Extracts from Anti-cancer Traditional Chinese Medicines

作　　者：董海燕[1] 邵敬伟[1] 王涛[1] 郭养浩[1] 严凌霞 

机构地区：[1]福州大学药物生物技术与工程研究所,福建福州350002

出　　处：《中药材》2008年第1期68-71,共4页Journal of Chinese Medicinal Materials

基　　金：福州大学校人才基金(HX2005-3)

摘　　要：目的:研究4种抗癌中药提取物对大鼠肝脏细胞色素P450 3A(CYP3A)酶在酶活性及mRNA水平的调节作用。方法:采用紫外分光光度法测定大鼠肝微粒体CYP450含量及CYP3A同工酶——红霉素N-脱甲基酶(ERD)的活性;采用反转录PCR(RT-PCR)技术检测CYP3A1、CYP3A2 mRNA的表达。结果:莪术、苍术、白术的提取物均可明显增加CYP450蛋白含量及CYP3A酶活性,而茯苓提取物无明显作用。在mRNA水平上,莪术、苍术、白术的提取物均能明显诱导CYP3A1的基因表达,茯苓提取物无明显作用;对于CYP3A2的基因表达,莪术和苍术的提取物均有明显的诱导作用,而白术和茯苓的提取物则有显著的抑制作用。结论:莪术、苍术、白术的提取物对CYP3A酶的调控均发生在酶活性水平和转录水平,而茯苓提取物对CYP3A酶仅存在转录水平的调控。4种抗癌中药提取物对CYP3A酶的诱导或抑制可能会影响其他药物的体内代谢。Objective: To study the effects of four kinds of extracts from anti-cancer Taditional Chinese Medicines on the activity and mRNA expression of CYP3A in rat′s liver.Methods: Rat′s liver microsomal cytochrome P450 and CYP3A isoemzymes——erythromycin N-demethylase(ERD) activities were determined by UV chromatography,the mRNA expression levels of CYP3A1 and CYP3A2 were detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Results: Extracts of Rhizoma Curcumae,Rhizoma Atractylodes and Rhizoma Atract...

关 键 词：抗癌中药 CYP3A RT-PCR 提取 

分 类 号：R285[医药卫生—中药学]