地黄水提液对2型糖尿病大鼠proinsulin基因表达的影响  

Effect of Rehmannia glutinosa Libosch Water Extraction on Gene Expression of Proinsulin in Type 2 Diabetes Mellitus Rats

作　　者：孟庆宇[1] 吕秀芳[2] 金秀东[3] 

机构地区：[1]牡丹江医学院药理学教研室,黑龙江牡丹江157011 [2]牡丹江医学院生物化学与分子生物学教研室,黑龙江牡丹江157011 [3]牡丹江医学院生理学教研室,黑龙江牡丹江157011

出　　处：《中药材》2008年第3期397-399,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:探讨地黄水提液治疗2型糖尿病(2-DM)的分子生物学机制。方法:以半定量RT-PCR法和SDS-聚丙烯酰胺凝胶电泳法分别检测胰岛素原(proinsulin)基因转录及翻译水平的表达。同时观察空腹血糖(FPG)、空腹胰岛素(FINS)的含量,计算胰岛素分泌指数(IS)、β细胞功能指数(HβCI)的变化,来研究不同剂量地黄水提液对上述各指标的影响。结果:与2-DM模型相比,地黄治疗组大鼠proinsulin基因mRNA和蛋白表达显著增高(P<0.01),FPG显著降低,FINS、IS、HβCI升高,差异均有显著性。结论:地黄水提液通过上调2-DM大鼠proinsulin基因mRNA和蛋白表达,可改善胰岛β细胞功能,降低2-DM大鼠血糖。Objective:To investigate the mechanisms of treating 2-DM by Rehmannia glutinosa Libosch water extraction(RGLE).Methods:The mRNA level of proinsulin in rats panreas tissue was examined by semi-quatitativa RT-PCR,and the protein was measured by SDS-PAGE.Results:The mRNA and protein expressions of proinsulin in RGLE group were higher than those of diabetic model group(P<0.01).The levels of FPG decreased.FINS,IS,HβCI increased(P<0.01).Conclusion:It may be the mechanism how the RGLE to decline high FPG and cure ...

关 键 词：地黄水提液 2型糖尿病 胰岛素原 基因表达 

分 类 号：R285.5[医药卫生—中药学]