彗星试验中全血法与分离淋巴细胞法的比较  

Comparison Research of Whole Blood Specimen and Abstracted Lymphocyte in Comet Assay

作　　者：段链[1] 杨静玉[1] 于海[1] 吴春福[1] 

机构地区：[1]沈阳药科大学药学院药理系,辽宁沈阳110016

出　　处：《癌变．畸变．突变》2004年第5期307-309,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的 :比较在彗星试验中全血法和分离淋巴细胞法的结果差异 ,为简化彗星试验方法提供实验依据。材料与方法 :重铬酸钾和乙醇腹腔注射给药 ,取全血或分离淋巴细胞 ,应用彗星实验法比较小鼠外周血液淋巴细胞的DNA损伤情况。结果 :在全血和分离淋巴细胞条件下均能检测到两种损伤剂所导致的细胞DNA损伤 ,且作用趋势相同。 结论 :采用全血进行彗星试验更为简便 。ed lymphocyte of mice after injecting K 2 Cr 2 O 7 and ethanol were used in comet assay to compare the degree of DNA damage. RESULTS: There was no difference of DNA damage of whole blood specimen and abstracted lymphocyte induced by those two noxious substances. CONCLUSION: Whole blood specimen is more convenient than abstracting lymphocytes in comet assay for detecting and screening of medicine on a large scale.

关 键 词：全血 淋巴细胞 重铬酸钾 乙醇 彗星试验 单细胞凝胶电泳 

分 类 号：R114[医药卫生—卫生毒理学]