应用^(32)P后标记法检测水中非挥发性有机物的DNA加合物  

Detection of DNA Adduct of Nonvolatile Organic Compounds in Tap Water by Using Nuclease P 1 Mediated ^(32)P-postlabeling Method

作　　者：唐非[1] 谷康定[1] 运珞珈[1] 张水兵[1] 汪亚州[1] 

机构地区：[1]华中科技大学同济医学院环境医学研究所,湖北武汉430030

出　　处：《癌变．畸变．突变》2004年第5期260-262,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家"九五"重点科技攻关计划项目(No.96-911-07-02-02)

摘　　要：背景与目的 :研究自来水中非挥发性有机物(NonvolatileOrganicCompounds,NOCs)与生物样品DNA形成DNA加合物的水平 ,尝试从分子水平来进一步验证水中NOCs的致突变作用及其机制。材料与方法 :应用XAD_2树脂吸附浓缩水中有机物的技术 ,结合 32P后标记法 ,对武汉东湖自来水中非挥发性有机物与体内外生物样品的DNA形成的DNA加合物进行了检测。结果 :自来水中的NOCs可直接与小牛胸腺DNA反应形成DNA加合物 ;小鼠经口染毒NOCs后 ,在其肝脏细胞可检出多个NOCs的DNA加合物。结论 :东湖自来水中的NOCs有直接损伤生物体内外DNA。BACKGROUND&AIM: The DNA adducts of the nonvolatile organic compounds(NOCs)in tap water were studied for further validating genotoxicity of NOC S and its mechanism at molecular level. MATERIAL AND METHODS: The sample of tap water derived from Donghu lake of Wuhan was collected and the nonvolatile organic compounds(NOCs)in water were concentrated on XAD_2resin.The DNA adducts of such NOCs were examined by using 32 P_postlabeling method. RESULTS: Some NOCs_DNA adducts could be found in biological samples in...

关 键 词：自来水 有机物 DNA加合物 

分 类 号：R114[医药卫生—卫生毒理学]