四脊裸胞壳Dh菌株液体培养条件下淀粉酶的产生及活性影响因子  

Production of Emericella quadriclineata Dh amylase in liquid culture and activity influential factors

作　　者：杨生伟[1] 朱建兰[1] 

机构地区：[1]甘肃农业大学草业学院植物保护系,甘肃兰州730070

出　　处：《甘肃农业大学学报》2006年第6期58-62,共5页Journal of Gansu Agricultural University

基　　金：甘肃省农牧厅生物技术专项(GNSW-2004-08)资助

摘　　要：对虫生真菌四脊裸胞壳(Emericella quadriclineata)Dh菌株产淀粉酶的条件及理化特性进行了研究.在孢子浓度为1.5×106个/mL的PD培养液中恒温(25±1)℃振荡(120 r/min)培养3.5 d,菌丝生物量最大达5.1 mg/mL,产淀粉酶量达272.42 U/mg;培养液初始pH4~6最有利淀粉酶的产生,产酶量为1 377.20~1 528.99 U/mg菌丝.淀粉酶活性在50℃,pH 6.0条件下最高.在pH4~7范围内20℃下处理20 min或在pH4~7范围内37℃下处理1 h酶活较为稳定,保持在65%以上.40℃下处理20 min酶活保持90%以上.60℃和70℃下处理20 min,酶活保持在50%以下.在70℃下处理80 min,剩余酶活仅为11.70%.一定浓度的Cu2+和Ba2+有利于酶活提高;同时Mg2+,Fe2+,Ca2+,NH4+对酶活性的抑制作用依次增强.Production condition and characteristics of E.quadriclineata Dh strain were studied in this paper.Shaking culture with the liquid containing 1.5×10^(6) connidia per millilitre for 3.5 d at the temperature of(25±1)℃ and 120 r/min resulted in max mycelial of 5.1 mg/mL and amylase yield of 272.42(U/mg).An initial range of pH 4～6 in the liquid culture was the most favorable to amylase production with the yields of 1377.20～1528.99 U/mg.The activity of E.quadriclineata Dh strain amylase was highest at 50 ℃ and ...

关 键 词：Dh菌 淀粉酶 产酶条件 酶活 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]