马麝朊蛋白(PrP)基因的克隆和序列分析  

Cloning and sequence analysis of prion protein (PrP) genes from musk deer

作　　者：陈豪泰[1] 吴润[1] 刘湘涛[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所农业部畜禽病毒学重点开放实验室,甘肃兰州730046

出　　处：《甘肃农业大学学报》2005年第3期292-296,共5页Journal of Gansu Agricultural University

基　　金：农业部畜禽病毒学重点开放实验室基金资助项目(2002-01);兰州兽医研究所所长基金资助(2004-2005)

摘　　要：从马麝的全血中提取基因组总DNA,用所设计引物以聚合酶链式反应扩增出细胞型朊蛋白(PrPC)基因,并克隆到pMD18-T载体。序列分析表明所克隆的马麝PrP基因片段大约为771bp,包含了朊蛋白基因的完整编码区序列,即包含在单一外显子内的完整开放阅读框,与国外报道的同科动物PrP基因序列基本相同。马麝PrP基因含5个短而富含G-C的元件,可编码5个八肽(九肽)重复Pro-His-Gly-Gly-Gly-Trp-Gly-Gln或Pro-Gln/His-Gly-Ala/Gly-Gly–Gly-Trp-Gly-Gln,其氨基酸序列含有24个氨基酸的N-端信号肽和23个氨基酸的C-端信号肽。与白尾鹿(Odocoileusvirginianus)和麋鹿(Cervuselaphus)的PrP基因相比,其核苷酸序列和推导氨基酸序列同源性分别为97.4%、97.9%和98.1%、97.7%。共发生15个碱基替换,其中10个为同义码替换,5个为异义突变,即G57A、S100N、N173S、T177N和M208I。Total genomic DNA were extracted from whole-blood of a musk deer. The PrP genes were amplified by polymerase chain reaction using a pair of primers, and then these genes were cloned into pMD 18-T vectors. The sequence showed that Prp gene of musk deer was 771 bp long, including complete coding region of Prp gene, which was a complete ORF contained within a single exon, and was basically same as the published gene sequences of animals in same family. The sequence of PrP gene contained five copies of a short ...

关 键 词：马麝 朊蛋白基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]