HP450基因的克隆及质粒载体的构建和鉴定  

作　　者：陈海英[1] 

机构地区：[1]广西医科大学药理教研室,广西南宁530021

出　　处：《国际医药卫生导报》2006年第1期4-6,共3页International Medicine and Health Guidance News

基　　金：国家自然科学资金(30360118)

摘　　要：目的HP450基因的克隆载体构建。方法用RT-PCR技术克隆HP450基因,T-A连接到PMD18-T载体,经菌液PCR,酶切鉴定.构建成PMD18-HP450重组质粒载体。结果测序显示完全正确,实现了HP450基因的克隆和质粒载体的构建。结论可以对重组成功的HP450基因进行体外表达和动物的体内应用.Objective To construct eukaryotic expression vector for HP450 Methods HP450 gene obtained by reverse transcriptase-polymerase chain reaction (RT-PCR) .It was cloned into pMD18-T vector using T/A clone method. ,The restriction digestion , bacterium liquid of PCR and sequencing were performed to evaluate the recombinant.The pMD18-HP450 vector was made. Results The synthetic DNA was of the correct sequence。 The HP450 gene had been cloned and the recombinant vector carrying HP450 had been constructed. Conclusio...

关 键 词：HP450基因 克隆 质粒载体 

分 类 号：R735.7[医药卫生—肿瘤]