含玉米pepc基因恢复系的MAS转育及其杂交稻的光合特性和杂种优势研究  

Photosynthetic Characteristics and Heterosis in Transgenic Hybrid Rice with Maize Phosphoenolpyruvate Carboxylase(pepc)Gene by MAS

作　　者：李季航[1] 向珣朝[1] 何立斌[1] 张楷正[1] 李平[1] 

机构地区：[1]四川农业大学水稻研究所

出　　处：《作物学报》2006年第12期1779-1787,共9页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2003AA212030;2004AA207110);教育部长江学者和创新团队发展计划(IRT0453)资助

摘　　要：用玉米pepc基因的特异性引物对转育pepc基因的后代材料进行分子标记辅助选育(MAS),成功地培育出与轮回亲本蜀恢881遗传相似度达97.09%～99.03%的改良蜀恢881,用此改良恢复系与3个不育系冈46A、776A、2480A形成组合。结果表明,(1)自行设计的pepc基因特异性引物能对基因进行准确的筛选,而且玉米pepc基因在新的遗传背景下能够稳定遗传和高水平表达;(2)含有pepc基因F1的PEPCase活力明显地高于对照,PEPCase活力与净光合速率(Pn)极显著正相关(0.6081**),表观量子效率、羧化效率等光合指标也显著好于对照;(3)含有pepc基因F1农艺性状表现各不相同,但千粒重和单株产量表现较为一致,且单株产量较对照平均增加41.48%;(4)776A、Line1和Line6的GCA较高,值得今后进一步考察与利用。Posterity materials carrying maize pepc gene has been successfully applied into marker-assisted selection(MAS)by using specific primer of maize pepc gene,and the improved Shuhui 881 with pepc gene has been obtained.The improved Shuhui 881 was similar to its backcross parents in genetic background with 97.09%-99.03%.Then rice sterile lines Gang 46A,776A,2480A as the female parents were crossed with several improved Shuhui 881 above.Some progress on photosynthetic characteristics and agronomic traits in hybri...

关 键 词：MAS 杂交稻 玉米PEPC基因 光合特性 高光效育种 

分 类 号：S511[农业科学—作物学]