次黄嘌呤维持小鼠卵母细胞减数分裂阻滞的时效性和可逆性研究  

Maintenance of meiotic arrest in mouse oocytes in vitro by hypoxanthine: the time-dependence and reversibility

作　　者：王子玉[1] 马所峰[2] 葛丽[2] 兰国成[2] 隋宏书[2] 陈红菊[2] 谭景和[2] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]山东农业大学动物科技学院,山东泰安271018

出　　处：《畜牧与兽医》2006年第12期7-10,共4页Animal Husbandry & Veterinary Medicine

基　　金：南京农业大学青年科技创新基金(Y200614)。

摘　　要：为提高体外成熟卵母细胞的发育能力,本实验采用卵泡内存在的减数分裂抑制剂次黄嘌呤(Hypoxanthine,HX)在体外维持小鼠GV期卵母细胞减数分裂阻滞,探讨了次黄嘌呤对卵母细胞减数分裂抑制作用的时效性、可逆性以及对卵丘扩展和解除抑制后的发育能力的影响。结果表明(1)4mmol/LHX维持减数分裂阻滞的作用具有时效性,GV%在18h时显著下降。(2)HX处理时间短于24h时,解除抑制后再成熟时卵母细胞的成熟率不受影响,抑制24h再成熟14h时成熟率仍可达86%。(3)HX处理会抑制卵丘扩展,解除抑制后再成熟时卵丘扩展程度跟抑制时间长短有关。(4)HX处理6h后,卵母细胞的孤雌激活率上升(42%vs20%,P<0.05),但胚胎的发育能力下降。这证明HX维持小鼠卵母细胞减数分裂阻滞的作用具有时效性和可逆性的特点,为建立提高体外成熟卵母细胞的发育能力的培养体系打下了基础。The developmental competence of in vitro matured oocytes is generally lower than that of the in vivo matured. Hypoxanthine, a physiological meiosis inhibitor in the follicular fluid, was used to inhibit nuclear maturation to improve the quality of oocytes matured in vitro. The results are summarized as follows: (1) HX (4 mmol/L) inhibited the meiotic maturation of mouse oocytes efficiently, but GV% decreased as time went by. (2) Maturation of oocytes was not affected when the HX exposure time was less than ...

关 键 词：次黄嘌呤 卵母细胞 减数分裂 小鼠 

分 类 号：S814.8[农业科学—畜牧学]