雨生红球藻β-胡萝卜素酮化酶(bkt)启动子功能分析(英文)  

FUNCTIONAL ANALYSIS OF THE PROMOTER OF BKT ENCODING BETA-CAROTENE KETOLASE IN HAEMATOCOCCUS PLUVIALIS

作　　者：魏炜[1] 梁成伟[1] 秦松[1] 

机构地区：[1]中国科学院海洋研究所

出　　处：《水生生物学报》2006年第6期747-751,共5页Acta Hydrobiologica Sinica

基　　金：the National Natural Science Foundationof China (No.30470156)andthe KeyInnovative Project of the Chinese Academyof Sciences(No.KZCX3-SW-215)

摘　　要：单细胞绿藻———雨生红球藻在逆境条件下积累大量的虾青素。β-胡萝卜素酮化酶(bkt)催化在β-胡萝卜素和玉米黄素的β-紫罗酮环C-4位引入酮基的反应,是虾青素合成过程中的关键酶。我们利用凝胶阻滞的方法研究雨生红球藻中bkt基因309bp(-617/-309)启动子区域的转录因子结合位点并发现在-396/-338的59bp探针存在特异的核蛋白结合位点。通过序列分析,发现此59bp区域并不包含TATA或者CAAT-box,而是存在对光、缺氧、p-香豆酸及激素反应的G-box。The unicellular green alga Haematococcus pluvialis accumulates a high-valuable astaxanthin under stress conditions. Beta-carotene ketolase (BKT), a key enzyme in astaxanthin biosynthesis in H. pluvialis, catalyzes the conversion of β-carotene to canthaxanthin and zeaxanthin to astaxanthin. Electrophoresis mobility shift assay (EMSA) was used in H. pluvialis to identify transcription factor binding sites within a 309 bp promoter region (-617/-309) of beta-carotene ketolase gene and a 59 bp sequence between -...

关 键 词：凝胶阻滞 雨生红球藻 bkt 启动子 转录因子结合位点 

分 类 号：Q943[生物学—植物学]