绿绒蒿自然杂交种及其亲本cpDNA trnL-trnF基因的遗传学分析  

Genetic Relationship between a Natural Hybrid Meconopsis× cookei(Papaveraceae)and Its Parents Based on cpDNA trnL-trnF Region Sequence

作　　者：袁长春[1] 何雪宝[1] 袁秋梅 施苏华[2] 

机构地区：[1]湛江师范学院生化学院,广东湛江524048 [2]中山大学生命科学学院基因工程教育部重点实验室,广东广州510275

出　　处：《云南植物研究》2007年第1期103-108,共6页Acta Botanica Yunnanica

基　　金：国家自然科学基金重点项目(30070053);广东省自然科学基金(04300850);湛江师范学院博士基金(Z0313)

摘　　要：对自然杂交种Meconopsis×cookei及其亲本红花绿绒蒿M.punicea和五脉绿绒蒿M.quintuplinervia的叶绿体DNAtrnL-trnF区进行了序列测定,所得序列的长度为957～961bp,其中M.×cookei的序列长度为960bp,红花绿绒蒿为961bp,五脉绿绒蒿为957bp。利用软件Clustal X对所得序列进行排序和碱基比较,排序后的序列长度为964bp,其中trnLintron为512bp,trnL3′exon为50bp,trnL-trn Fintergenic spacer(IGS)为361bp,还包括41bp的trnF5′端片段。整个trnL-trnF区序列共有25个变异位点,其中杂交种M.×cookei与红花绿绒蒿具有相同碱基的位点有21个(占84%),M.×cookei与五脉绿绒蒿具有相同碱基的位点仅有1个(占4%),余下3个位点(占12%)中,M.×cookei的碱基与两个亲本均不相同。分析结果表明,杂交种M.×cookei的叶绿体基因trnL-trnF来自红花绿绒蒿,根据质体细胞质遗传的规律,从而推测红花绿绒蒿为该杂交种的母本,五脉绿绒蒿为其父本。The Chloroplast DNA trnL-trnF region sequences from a natural hybrid species Meconopsis×cookei and its parents M.punicea and M.quintuplinervia were obtained by using direct sequencing method.The sequence length of trnL-trnF region is 960 bp for M.×cookei,961 bp for M.punicea,and 957 bp for M.quintuplinervia.The sequences were aligned by the software Clustal X,and then the bases per locus were compared by using the software with manual method.The aligned sequence length is 964 bp,of which trnL intron is 512 ...

关 键 词：绿绒蒿杂交种 红花绿绒蒿 五脉绿绒蒿 TRNL-TRNF 

分 类 号：Q943[生物学—植物学]