镉对去卵巢大鼠子宫增重及血清性激素水平的影响  

Effects of Cadmium on Uterotrophic Assay and Serum Sexual Hormones Level in Ovariectomized Rats

作　　者：杨劲松[1] 张文昌[1] 陈昱[1] 汪家犁 林炜[1] 贾艳[1] 

机构地区：[1]福建医科大学公共卫生学院职业与环境卫生学系,福建福州350004

出　　处：《环境与健康杂志》2007年第3期146-148,共3页Journal of Environment and Health

基　　金：福建省自然科学基金资助项目(F0210002)

摘　　要：目的研究镉对去卵巢大鼠子宫增重效应及血清性激素水平的影响。方法28天龄健康雌性SD大鼠40只,切除双侧卵巢后按体重分层,分为4组,即阴性对照组(超纯水)、低镉组(0.12mg/kg)、高镉组(1.2mg/kg)、阳性对照组(0.03mg/kg的17β-雌二醇)。各组大鼠均按1ml/kg腹腔注射染毒,每天1次,连续3d。染毒结束后称子宫湿重,计算子宫脏器系数;采用放免法测定血清雌二醇(E2)、孕酮(P4)、卵泡刺激素(FSH)、黄体生成素(LH)水平。结果高镉组、阳性对照组子宫湿重与子宫脏器系数均高于阴性对照组,差异有统计学意义(P<0.05,P<0.01)。与阴性对照组比较,高镉组大鼠血清E2与P4水平降低,差异有统计学意义(P<0.05);阳性对照组血清E2与P4水平升高,差异有统计学意义(P<0.01);低镉组大鼠血清FSH与LH水平降低,差异有统计学意义(P<0.05,P<0.01);阳性对照组血清LH水平升高,差异有统计学意义(P<0.01)。结论在本实验条件下,镉具有子宫增重效应,并能通过非性腺组织发挥内分泌干扰作用。Objective To study estrogenicity effect of cadmium with uterotrophic assay and to study the effect of cadmium on serum sexual hormones level in ovariectomized rats.Methods 40 ovariectomized SD rats aged 28 days were divided in to groups and treated respectively with cadmium by intraperitoneal injection at the doses of 0,0.12 and 1.2 mg/kg per day and estrogen of 0.03 mg/kg(positive control)per day for 3 consecutive days.Uteruses wet weight were measured and serum E2,P4,FSH,LH were determined.Results Compare...

关 键 词：金属 重 镉 雌激素类 去卵巢大鼠 子宫增重试验 

分 类 号：R114[医药卫生—卫生毒理学]