HPLC法和FPIA法测定茶碱血清浓度的相关性  

Research on relativity between serum theophylline concentrations determined by HPLC and by FPIA

作　　者：李俊杰[1] 杨杲岚[1] 陈琳[1] 

机构地区：[1]暨南大学附属第一医院临床药理室,广东广州510630

出　　处：《暨南大学学报（自然科学与医学版）》2006年第2期230-232,238,共4页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：目的:建立快速测定茶碱血清浓度的高效液相色谱(HPLC)法,研究应用此法和常规的荧光偏振免疫分析(FPIA)法测定结果的相关性。方法:分别用HPLC和FPIA法测定58份茶碱血清样品,比较两种方法的测定结果。结果:HPLC法(Y)和FPIA法(X)测定的茶碱血清浓度的回归方程为Y=0.282 5+1.064X(r=0.988 1)。r的t检验显示两种方法测定结果之间的直线相关关系有显著意义(P<0.001)。结论:用HPLC法和FPIA法测定的茶碱血清浓度具有良好的相关性;与FPIA法相比,该HPLC法不仅同样具有快速、简便和准确的特点,而且检测成本较低,也可作为监测茶碱血清浓度的常规方法。Aim: To establish a rapid HPLC method determining serum theophylline concentration and research the relativity of results determined by HPLC and by routine FPIA.Methods:Serum theophylline concentrations of 58 samples were determined by HPLC and by FPIA separately,and the results were compared.Results: The regression(equation) of serum theophylline concentrations determined by HPLC(Y) and by FPIA(X) was Y=0.282 5+1.064X(r=0.988 1).The t test of correlation coefficient showed significant linear correlation be...

关 键 词：高效液相色谱 荧光偏振免疫分析 茶碱血清浓度 

分 类 号：R96[医药卫生—药理学]