体外快速筛选抗HIV药物的一种新方法  

A novel method for rapid screening of anti-HIV drugs in vitro

作　　者：曾祥凤[1] 曾耀英[1] 李海仙[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2006年第2期233-238,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金："十五"国家科技攻关计划中医药治疗艾滋病专项基金(2004BA719A09-03)

摘　　要：目的:介绍一种体外快速筛选抗H IV药物的新方法。方法:采用荧光染料钙荧光素(Cal-ce in-AM)标记H IV感染的慢性H9细胞(H9/H IV-1ⅢB)与不同稀释浓度的药物作用后,与靶细胞MT-2细胞混合培养,2 h后用荧光显微镜观察MT-2和H9/H IV-1ⅢB融合细胞的变化;孵育3 d后用MTT法检测药物对H IV感染细胞的保护作用和H IV-1p24抗原试剂盒检测药物作用后的细胞培养上清p24抗原含量的变化。结果:药物(双黄连粉针剂)能够抑制MT-2和H9/H IV-1ⅢB细胞融合(双黄连粉针剂抑制融合的EC50为3.18 mg/L,选择指数SI大于314.5);能够保护H IV感染细胞的死亡(EC50为10.59 mg/L,SI大于94.43)和减少H IV-1p24抗原的表达(EC50为23.29 mg/L,SI大于42.9)。结论:运用此方法4~6 h即可初步判断药物是否具有抗H IV的作用,此方法可简便直观,敏感快速进行抗H IV药物的筛选。Aim: To introduce a new method for screening anti-HIV drugs in vitro.Methods:Calcein-AM stained H9/HIV-1ⅢB cells were treated with serially diluted compounds,then co-cultured with target cells MT-2,and HIV-1 mediated cell fusion was observed under a fluorescent microscope.The protective effect of compounds on HIV-1-infected cells was determined by MTT.Viral replication was evaluated by measuring the level of p24 antigen in culture supernatants by ELISA.Results: Compounds(SHL injection) could suppress the fu...

关 键 词：药物筛选模型 艾滋病 人免疫缺陷病毒 

分 类 号：R965.1[医药卫生—药理学]