一株富含类胡萝卜素光合细菌的分离鉴定  

Isolation and identification of a strain of photosynthetic bacteria with high-yield production of carotenoid pigments

作　　者：孙勇[1] 谢数涛[1] 杨泽民[1] 许忠能[1] 

机构地区：[1]暨南大学水生生物研究所,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2006年第1期145-150,共6页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：从不同生物环境中分离紫色非硫菌,测定其类胡萝卜素含量,筛选出富含类胡萝卜素的菌株.对该菌株的形态学特征、碳氮源利用情况、最适生长pH值及盐度等生物学特征进行研究;利用PCR技术扩增其16S rRNA基因,测序后进行16S rRNA基因序列分析,研究其分类地位.结果:分离自暨南大学明湖底泥的菌株R2,在所分离的8株紫色非硫菌中,类胡萝卜素质量分数最高,为4.83 mg/g(干菌体),该菌株符合红游动菌属(Rhodoplanes)的生物学特性,其最适生长pH为7.0,盐度为0.2%;16S rRNA基因的分子系统学分析表明,该菌株与红游动菌属的Rhodoplanessp.HA17相似性为99.4%.可确定筛选出一株高产类胡萝卜素的菌株,该菌株属于红游动菌属(Rhodoplanes).Purple nonsulfur bacteria were isolated from different habitats.The contents of carotenoid pigments in their dry cells were measured and a strain with high-yield production was selected.Its biological characteristics including morphology,the ability to utilize different carbon and nitrogen sources,the optimal pH value and salt concentration for growth were studied.The 16S rRNA gene was amplified and sequenced.The sequence was compared with those of related species using ClustalX.Strain R2 isolated from the ...

关 键 词：类胡萝卜素 紫色非硫菌 16SrRNA 红游动菌属 

分 类 号：Q93-33[生物学—微生物学]